Ontology highlight
ABSTRACT:
SUBMITTER: Puligedda RD
PROVIDER: S-EPMC6512912 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Puligedda Rama Devudu RD Sharma Rashmi R Al-Saleem Fetweh H FH Kouiavskaia Diana D Velu Arul Balaji AB Kattala Chandana Devi CD Prendergast George C GC Lynch David R DR Chumakov Konstantin K Dessain Scott K SK
mAbs 20190222 3
Hybridoma methods for monoclonal antibody (mAb) cloning are a mainstay of biomedical research, but they are hindered by the need to maintain hybridomas in oligoclonal pools during antibody screening. Here, we describe a system in which hybridomas specifically capture and display the mAbs they secrete: On-Cell mAb Screening (OCMS™). In OCMS™, mAbs displayed on the cell surface can be rapidly assayed for expression level and binding specificity using fluorescent antigens with high-content (image-b ...[more]