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Differential and tissue-specific activation pattern of the AtPROPEP and AtPEPR genes in response to biotic and abiotic stress in Arabidopsis thaliana.

ABSTRACT: In Arabidopsis thaliana AtPEPR1 and AtPEPR2 act as the receptors for the endogenous AtPROPEP-derived Pep peptides and subsequently initiate defense-signaling cascades. In the previous work,⁹ the expression pattern of the genes encoding the PEPR receptors and the AtPROPEP peptide precursor proteins was studied using promoter-GUS reporter constructs. Here, using the same constructs to study their expression pattern under biotic and abiotic stress, including AtPep1, flg22, methyl jasmonate (MeJA), and NaCl treatments, we observed that in response to AtPep1 and flg22, the activation of AtPEPR1 promoter was different from AtPEPR2. We also found that these promoters were differentially activated in response to NaCl. Remarkably, we showed that it is possible to classify the genes of the AtPROPEP family, based on the response of their promoters to the various stimuli employed: thus, we classify AtPROPEP1 in one group; AtPROPEP2 and AtPROPEP3 in a second group; AtPROPEP4, AtPROPEP7 and AtPROPEP8 in a third group and AtPROPEP5 in a fourth group. Our finding, confirm non-redundant roles among the members of the AtPROPEP family and their corresponding receptors.

SUBMITTER: Safaeizadeh M

PROVIDER: S-EPMC6512929 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Abiotic and biotic stress can have a detrimental impact on plant growth and productivity. Hence, there is a substantial demand for key factors of stress responses to improve yield stability of crops. Members of the poly(ADP-ribose)polymerase (PARP) protein family, which post-translationally modify (PARylate) nuclear proteins, have been suggested as such universal determinants of plant stress responses. A role under abiotic stress has been inferred from studies in which a genetic or, more commonly, pharmacological inhibition of PARP activity improved the performance of stressed plants. To further elucidate the role of PARP proteins under stress, T-DNA knockout mutants for the three Arabidopsis thaliana PARP genes were subjected to drought, osmotic, salt, and oxidative stress. To exclude a functional redundancy, which was indicated by a transcriptional upregulation of the remaining parp genes, a parp triple mutant was generated. Surprisingly, parp mutant plants did not differ from wild type plants in any of these stress experiments, independent from the number of PARP genes mutated. The parp triple mutant was also analyzed for callose formation in response to the pathogenassociated molecular pattern flg22. Unexpectedly, callose formation was unaltered in the mutant, albeit pharmacological PARP inhibition robustly blocked this immune response, confirming previous reports. Evidently, pharmacological inhibition appears to be more robust than the abolition of all PARP genes, indicating the presence of so-far undescribed proteins with PARP activity. This was supported by the finding that protein PARylation was not absent, but even increased in the parp triple mutant. Candidates for novel PARP-inhibitor targets may be found in the SRO protein family. These proteins harbor a catalytic PARP-like domain and are centrally involved in stress responses. Molecular modeling analyses, employing animal PARPs as templates, indeed indicated a capability of the SRO proteins RCD1 and SRO1 to bind nicotinamide-derived inhibitors. Collectively, the results of our study suggest that the stress-related phenotypes of parp mutants are highly conditional, and they call for a reconsideration of PARP inhibitor studies. In the context of this study, we also propose a unifying nomenclature of PARP genes and parp mutants, which is currently highly inconsistent and redundant.

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Differential and tissue-specific activation pattern of the AtPROPEP and AtPEPR genes in response to biotic and abiotic stress in Arabidopsis thaliana.

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