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Phenotypical characterisation of a putative ?-amino acid transaminase in the yeast Scheffersomyces stipitis.


ABSTRACT: Phylogenetic analysis of class III transaminases in the budding yeasts Lachancea kluyveri, Saccharomyces cerevisiae and Scheffersomyces stipitis identified a hitherto uncharacterised Sch. stipitis transaminase encoded by the PICST_54153 gene, which clustered with previously described ?-amino butyric acid (GABA) and ?-alanine transaminases. Deletion of the PICST_54153 gene in Sch. stipitis resulted in a complete loss in the utilisation of ?-alanine and ?-ureidopropionic acid as nitrogen sources, while growth on 1,3-diaminopropane displayed a significant lag phase compared to the wild-type control. It was therefore concluded that the Sch. stipitis PICST_54153 gene likely encodes a ?-alanine transaminase. However, minor growth defects when 1,4-diaminobutane or 1,5-diaminopentane was provided as the nitrogen source suggested that the Picst_54153 transaminase may also participate in the catabolism of other diamine-derived ?-amino acids. Unexpectedly, the ?picst_54153 deletion mutant failed to grow on solid minimal medium in the presence of 5 mM ?-alanine even if a preferred nitrogen source was provided.

SUBMITTER: Linder T 

PROVIDER: S-EPMC6514085 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Phenotypical characterisation of a putative ω-amino acid transaminase in the yeast Scheffersomyces stipitis.

Linder Tomas T  

Archives of microbiology 20181206 2


Phylogenetic analysis of class III transaminases in the budding yeasts Lachancea kluyveri, Saccharomyces cerevisiae and Scheffersomyces stipitis identified a hitherto uncharacterised Sch. stipitis transaminase encoded by the PICST_54153 gene, which clustered with previously described γ-amino butyric acid (GABA) and β-alanine transaminases. Deletion of the PICST_54153 gene in Sch. stipitis resulted in a complete loss in the utilisation of β-alanine and β-ureidopropionic acid as nitrogen sources,  ...[more]

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