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Structural Identification of Antibacterial Lipids from Amazonian Palm Tree Endophytes through the Molecular Network Approach.


ABSTRACT: A library of 197 endophytic fungi and bacteria isolated from the Amazonian palm tree Astrocaryum sciophilum was extracted and screened for antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). Four out of five antibacterial ethyl acetate extracts were also cytotoxic for the MRC-5 cells line. Liquid chromatography coupled to tandem mass spectrometry (UPHLC-HRMS/MS) analyses combined with molecular networking data processing were carried out to allow the identification of depsipeptides and cyclopeptides responsible for the cytotoxicity in the dataset. Specific ion clusters from the active Luteibacter sp. extract were also highlighted using an MRSA activity filter. A chemical study of Luteibacter sp. was conducted leading to the structural characterization of eight fatty acid exhibiting antimicrobial activity against MRSA in the tens of µg/mL range.

SUBMITTER: Barthelemy M 

PROVIDER: S-EPMC6514718 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Structural Identification of Antibacterial Lipids from Amazonian Palm Tree Endophytes through the Molecular Network Approach.

Barthélemy Morgane M   Elie Nicolas N   Pellissier Léonie L   Wolfender Jean-Luc JL   Stien Didier D   Touboul David D   Eparvier Véronique V  

International journal of molecular sciences 20190424 8


A library of 197 endophytic fungi and bacteria isolated from the Amazonian palm tree <i>Astrocaryum sciophilum</i> was extracted and screened for antibacterial activity against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). Four out of five antibacterial ethyl acetate extracts were also cytotoxic for the MRC-5 cells line. Liquid chromatography coupled to tandem mass spectrometry (UPHLC-HRMS/MS) analyses combined with molecular networking data processing were carried out to allow the  ...[more]

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