Ontology highlight
ABSTRACT: Aims
Neonatal hypoxia-ischemia (H/I) results in gray and white matter injury, characterized by neuronal loss, failure of neural network formation, retarded myelin formation, and abnormal accumulation of oligodendrocyte progenitor cells (OPCs). These changes lead to severe neurological deficits and mortality. Sublethal hypoxic preconditioning (HPC) can protect the developing brain against H/I. However, limited evidence is available concerning its effect on white matter injury.Methods
In this study, P6 neonatal Sprague-Dawley rats were subjected to normoxic (21% O2 ) or HPC (7.8% O2 ) for 3 hours followed 24 hours later by H/I brain injury. Neurological deficits were assessed by gait, righting reflex, foot fault, and Morris water maze tests. Compound action potential of the corpus callosum was recorded 35 days after surgery, and the correlation between axon myelination and neurological function was determined.Results
Hypoxic preconditioning significantly attenuated H/I brain injury at 7 days and remarkably improved both sensorimotor and cognitive functional performances up to 35 days after H/I. HPC-afforded improvement in long-term neurological outcomes was attributable, at least in part, to restoration of the differentiation and maturation capacity in oligodendrocyte progenitor cells, amelioration of microglia/macrophage activation and neuroinflammation, and continuation of brain development after H/I.Conclusions
Hypoxic preconditioning restores white matter repair, development, and functional integrity in developing brain after H/I brain injury.
SUBMITTER: Xu MY
PROVIDER: S-EPMC6515700 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Xu Ming-Yue MY Wang Yang-Fan YF Wei Peng-Ju PJ Gao Yan-Qin YQ Zhang Wen-Ting WT
CNS neuroscience & therapeutics 20190128 6
<h4>Aims</h4>Neonatal hypoxia-ischemia (H/I) results in gray and white matter injury, characterized by neuronal loss, failure of neural network formation, retarded myelin formation, and abnormal accumulation of oligodendrocyte progenitor cells (OPCs). These changes lead to severe neurological deficits and mortality. Sublethal hypoxic preconditioning (HPC) can protect the developing brain against H/I. However, limited evidence is available concerning its effect on white matter injury.<h4>Methods< ...[more]