Dataset Information

Isolation of cellulase enzyme from brown garden snail (Cornu aspersum) for the saccharification of waste paper materials.

ABSTRACT: Garden snails (Cornu aspersum) have been sacrificed by drowning the snails overnight in water. The visceral organs (inside the shell organs) have been separated from the foot as well as the shell and homogenized using tris-HCl buffer, pH 5. The homogenate of visceral organs was dialysed in distilled water at 4?°C for 18?h where after the dialysed material was used to bio-convert the cellulose component of various waste paper materials into fermentable sugars such as glucose. Saccharification of the waste cellulose materials was performed with the extracted snail cellulase during ten consecutive incubation periods of 2?h each. The amount of sugars produced during cellulase action on waste cellulose was determined by the dinitrosalicylic acid (DNS) method. All incubations were performed in triplicate and the percent saccharification of each paper material was determined as a fraction of the paper material exposed to cellulase action. •Cellulase extracted from brown garden snail•Saccharification of waste paper using garden snail cellulase.

SUBMITTER: Ndlovu TM

PROVIDER: S-EPMC6517372 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Isolation of cellulase enzyme from brown garden snail (<i>Cornu aspersum</i>) for the saccharification of waste paper materials.

Ndlovu T M TM Van Wyk J P H JPH

MethodsX 20190424

Garden snails (<i>Cornu aspersum</i>) have been sacrificed by drowning the snails overnight in water. The visceral organs (inside the shell organs) have been separated from the foot as well as the shell and homogenized using tris-HCl buffer, pH 5. The homogenate of visceral organs was dialysed in distilled water at 4 °C for 18 h where after the dialysed material was used to bio-convert the cellulose component of various waste paper materials into fermentable sugars such as glucose. Saccharificat ...[more]

PMID: 31193059

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Project description:This study is the first on the genetics of invasive populations of one of the most widely spread land mollusc species known in the world, the "Brown Snail" Cornu aspersum aspersum. Deliberately or accidentally imported, the species has become recently a notorious pest outside its native Mediterranean range. We compared the spatial structure and genetic variability of invasive (America, Oceania, South Africa) versus native populations using five microsatellite loci and mitochondrial (Cyt b and 16S rRNA) genes as a first step towards (i) the detection of potential source populations, and (ii) a better understanding of mechanisms governing evolutionary changes involved in the invasion process. Results based on multivariate analysis (Discriminant Analysis of Principal Components), Bayesian statistical inference (Clustering, Approximate Bayesian Computation) and demographic tests allowed a construction of the introduction pathways of the species over recent centuries. While emigrants originated from only one of the two native lineages, the West one, the most likely scenario involved several introduction events and "source switching" comprising (i) an early stage (around 1660) of simultaneous introductions from Europe (France, Spain) towards Oceania (New Zealand) and California, (ii) from the early 18(th) century, a second colonization wave from bridgehead populations successfully established in California, (iii) genetic admixture in invasive areas where highly divergent populations came into contact as in New Zealand. Although these man-made pathways are consistent with historical data, introduction time estimates suggest that the two putative waves of invasion would have occurred long before the first field observations recorded, both in America and in Oceania. A prolonged lag period as the use of an incorrect generation time could explain such 100-150 years discrepancy. Lastly, the contrasting patterns of neutral genetic signal left in invasive populations are discussed in light of possible ways of facing novel environments (standing genetic variation versus new mutation).

Dataset Information

Isolation of cellulase enzyme from brown garden snail (Cornu aspersum) for the saccharification of waste paper materials.

Publications

Isolation of cellulase enzyme from brown garden snail (<i>Cornu aspersum</i>) for the saccharification of waste paper materials.

Similar Datasets

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