Dataset Information

Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins.

ABSTRACT:

Introduction

Stem cell therapy with mesenchymal stem cells (MSCs) has been widely used in many clinical trials, and therapy with MSC sheets shows promise for patients. However, there are few reports characterizing MSC sheets. In the present study, the properties of MSC sheets derived from bone marrow, adipose tissue, and umbilical cord were evaluated.

Methods

Cell sheets were fabricated with MSCs from different tissue origins in temperature-responsive cell culture dishes with and without pre-coating of fetal bovine serum (FBS). MSC adhesion behavior in the culture dish was observed. Secretion of cytokines related to cell proliferation and immune regulation from MSC sheets was investigated by ELISA. The adhesion properties of the MSC sheets were investigated by time-lapse microscopy.

Results

Different cell adhesion and proliferation rates in temperature-responsive cell culture dishes were observed among the three types of MSCs. FBS pre-coating of the dishes enhanced cell attachment and proliferation in all cell types. Harvested cell sheets showed high attachment capacity to tissue culture polystyrene dish surfaces.

Conclusions

MSC sheets can be fabricated from MSCs from different tissue origins using temperature-responsive cell culture dishes. The fabricated MSC sheets could be useful in cell transplantation therapies by choosing appropriate types of MSCs that secrete therapeutic cytokines for the targeted diseases.

SUBMITTER: Nakao M

PROVIDER: S-EPMC6517796 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Publications

Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins.

Nakao Mitsuyoshi M Inanaga Daimu D Nagase Kenichi K Kanazawa Hideko H

Regenerative therapy 20190510

<h4>Introduction</h4>Stem cell therapy with mesenchymal stem cells (MSCs) has been widely used in many clinical trials, and therapy with MSC sheets shows promise for patients. However, there are few reports characterizing MSC sheets. In the present study, the properties of MSC sheets derived from bone marrow, adipose tissue, and umbilical cord were evaluated.<h4>Methods</h4>Cell sheets were fabricated with MSCs from different tissue origins in temperature-responsive cell culture dishes with and ...[more]

PMID: 31193157

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Dataset Information

Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins.

Introduction

Methods

Results

Conclusions

Publications

Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins.

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