Repeated Phenotypic Evolution by Different Genetic Routes in Pseudomonas fluorescens SBW25.

ABSTRACT: Repeated evolution of functionally similar phenotypes is observed throughout the tree of life. The extent to which the underlying genetics are conserved remains an area of considerable interest. Previously, we reported the evolution of colony switching in two independent lineages of Pseudomonas fluorescens SBW25. The phenotypic and genotypic bases of colony switching in the first lineage (Line 1) have been described elsewhere. Here, we deconstruct the evolution of colony switching in the second lineage (Line 6). We show that, as for Line 1, Line 6 colony switching results from an increase in the expression of a colanic acid-like polymer (CAP). At the genetic level, nine mutations occur in Line 6. Only one of these-a nonsynonymous point mutation in the housekeeping sigma factor rpoD-is requ

SUBMITTER: Gallie J 

PROVIDER: S-EPMC6519391 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature