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Impact of Aging and HIV Infection on the Function of the C-Type Lectin Receptor MINCLE in Monocytes.


ABSTRACT: Both aging and HIV infection are associated with an enhanced pro-inflammatory environment that contributes to impaired immune responses and is mediated in part by innate immune pattern-recognition receptors. MINCLE is a C-type lectin receptor that recognizes trehalose-6,6'-dimycolate or "cord factor," the most abundant glycolipid in Mycobacterium tuberculosis. Here, we evaluated MINCLE function in monocytes in a cohort of HIV-infected and uninfected young (21-35 years) and older adults (?60 years) via stimulation of peripheral blood mononuclear cells with trehalose-6,6-dibehenate, a synthetic analog of trehalose-6,6'-dimycolate and measurement of cytokine production (interleukin [IL]-10, IL-12, IL-6, tumor necrosis factor-?) by multicolor flow cytometry. Our studies show an age- and HIV-associated increase in cytokine multifunctionality of monocytes both at the population and single cell level that was dominated by IL-12, IL-10, and IL-6. These findings provide insight into the host response to M. tuberculosis and possible sources for the pro-inflammatory environment seen in aging and HIV infection.

SUBMITTER: Zapata HJ 

PROVIDER: S-EPMC6521921 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Impact of Aging and HIV Infection on the Function of the C-Type Lectin Receptor MINCLE in Monocytes.

Zapata Heidi J HJ   Van Ness Peter H PH   Avey Stefan S   Siconolfi Barbara B   Allore Heather G HG   Tsang Sui S   Wilson Jean J   Barakat Lydia L   Mohanty Subhasis S   Shaw Albert C AC  

The journals of gerontology. Series A, Biological sciences and medical sciences 20190501 6


Both aging and HIV infection are associated with an enhanced pro-inflammatory environment that contributes to impaired immune responses and is mediated in part by innate immune pattern-recognition receptors. MINCLE is a C-type lectin receptor that recognizes trehalose-6,6'-dimycolate or "cord factor," the most abundant glycolipid in Mycobacterium tuberculosis. Here, we evaluated MINCLE function in monocytes in a cohort of HIV-infected and uninfected young (21-35 years) and older adults (≥60 year  ...[more]

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