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M2 macrophages promote NSCLC metastasis by upregulating CRYAB.


ABSTRACT: The mechanism by which tumor-associated macrophages (TAMs) affect cancer progression is not fully understood. This study developed a microfluidic-based co-culture device to mimic the tumor microenvironment to assess TAM effects on invasion and metastasis in NSCLC. The results showed lung carcinoma cells could cause macrophages to show the M2 (a TAM-like) phenotype, and these M2 macrophages promoted lung cancer cell EMT and invasion. Proteomic analysis by the iTRAQ quantitation strategy and GO ontology of the cancer cells indicated that ?B-Crystallin (CRYAB) might be involved in this process. Further, we confirmed the role of CRYAB in cancer invasion and metastasis through cell and animal experiments, as well as human cancer tissue assessment. Overall, we demonstrated that M2 macrophages promote malignancy in lung cancer through the EMT by upregulating CRYAB expression and activating the ERK1/2/Fra-1/slug signaling pathway.

SUBMITTER: Guo Z 

PROVIDER: S-EPMC6522541 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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M2 macrophages promote NSCLC metastasis by upregulating CRYAB.

Guo Zhe Z   Song Jing J   Hao Junxia J   Zhao Hui H   Du Xiaohui X   Li Encheng E   Kuang Yanbin Y   Yang Fuquan F   Wang Wei W   Deng Jiong J   Wang Qi Q  

Cell death & disease 20190516 6


The mechanism by which tumor-associated macrophages (TAMs) affect cancer progression is not fully understood. This study developed a microfluidic-based co-culture device to mimic the tumor microenvironment to assess TAM effects on invasion and metastasis in NSCLC. The results showed lung carcinoma cells could cause macrophages to show the M2 (a TAM-like) phenotype, and these M2 macrophages promoted lung cancer cell EMT and invasion. Proteomic analysis by the iTRAQ quantitation strategy and GO on  ...[more]

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