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Sonography and sonoelastography in the detection of malignancy in superficial lymph nodes of dogs.

ABSTRACT: BACKGROUND:The clinical applicability of sonography and sonoelastography (SOE) in the detection of lymph node malignancy in dogs has not been established. OBJECTIVES:To compare sonographic and sonoelastographic findings between malignant and benign superficial lymph nodes and to evaluate the diagnostic performance of those methods. ANIMALS:One-hundred sixteen lymph nodes of 54 dogs. METHODS:A prospective observational study was used to investigate sonographic features and elasticity scores of malignant and benign superficial lymph nodes. Lymph nodes were categorized as malignant or benign according to cytology or histopathology. Quantitative variables were compared using Student's unpaired t test. Prevalence of categorical variables was compared using nonparametric Mann-Whitney U test. Diagnostic performance was calculated by receiver-operating characteristic analysis. RESULTS:Forty-nine malignant and 67 benign lymph nodes were included. Malignant nodes had larger long axis (LA; P?=?.0002), short axis (SA; P?

SUBMITTER: Belotta AF

PROVIDER: S-EPMC6524127 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Sonography and sonoelastography in the detection of malignancy in superficial lymph nodes of dogs.

Belotta Alexandra F AF Gomes Marcela C MC Rocha Noeme S NS Melchert Alessandra A Giuffrida Rogério R Silva Jeana P JP Mamprim Maria J MJ

Journal of veterinary internal medicine 20190318 3

<h4>Background</h4>The clinical applicability of sonography and sonoelastography (SOE) in the detection of lymph node malignancy in dogs has not been established.<h4>Objectives</h4>To compare sonographic and sonoelastographic findings between malignant and benign superficial lymph nodes and to evaluate the diagnostic performance of those methods.<h4>Animals</h4>One-hundred sixteen lymph nodes of 54 dogs.<h4>Methods</h4>A prospective observational study was used to investigate sonographic feature ...[more]

PMID: 30883935

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Project description:INTRODUCTION:In southern European countries, multicentric lymphoma and leishmaniosis are the main differential diagnoses in dogs presented with generalized lymphadenomegaly. The cytological examination is in some cases inconclusive and polymerase chain reaction (PCR) for antigen receptor rearrangement (PARR) has become a common method to confirm or rule out a lymphoproliferative neoplasia. According to the literature, leishmaniosis may lead to clonal arrangements and therefore to a false diagnosis of lymphoma, but this assumption is made from a single leishmania infected dog. Therefore, the objective of this study was to prospectively evaluate results from PARR in dogs with lymphadenomegaly due to clinical leishmaniosis at the moment of diagnosis. MATERIALS AND METHODS:31 dogs with a diagnosis of leishmaniosis based on the LeishVet guidelines were included in the study. Samples from enlarged lymph nodes were taken for cytological examination, clonality testing and Leishmania infantum PCR. RESULTS:All 31 dogs had medium to high positive antibody titers against Leishmania spp. and 30/31 had a positive Leishmania PCR from the lymph node. A polyclonal arrangement for B cells (immunoglobulin heavy chain gene) and T cells (T-cell receptor gamma chain gene) antigen receptors was found in 28/31 dogs. Two out of 31 dogs showed a monoclonal arrangement for Ig with high (1:2) and low (1:7) polyclonal background respectively; and one of the 31 dogs showed a monoclonal arrangement for T cell receptor with low (1:3) polyclonal background. CONCLUSION:Infections with Leishmania infantum resulted in clonal rearrangement, and therefore in a possible false diagnosis of lymphoma, in 3 out of 31 dogs (9.7%). Although, PARR is a useful method to differentiate lymphoma from reactive lymphoid hyperplasia in dogs with leishmaniosis, mono-/biclonal results should be interpreted carefully, especially in the presence of any degree of polyclonal background, and together with other clinicopathological findings.

Project description:BackgroundAlveolar (AE) and cystic echinococcosis (CE) in humans are caused by the metacestode of the tapeworms Echinococcus multilocularis and Echinococcus granulosus sensu lato (s.l.). Immunohistochemistry with the monoclonal antibodies (mAb) Em2G11, specific for AE, and the mAb EmG3, specific for AE and CE, is an important pillar of the histological diagnosis of these two infections. Our aim was to further evaluate mAb EmG3 in a diagnostic setting and to analyze in detail the localization, distribution, and impact of small particles of Echinococcus multilocularis (spems) and small particles of Echinococcus granulosus s.l. (spegs) on lymph nodes.Methodology/principal findingsWe evaluated the mAb EmG3 in a cohort of formalin-fixed, paraffin embedded (FFPE) specimens of AE (n = 360) and CE (n = 178). These samples originated from 156 AE-patients and 77 CE-patients. mAb EmG3 showed a specific staining of the metacestode stadium of E. multilocularis and E. granulosus s.l. and had a higher sensitivity for spems than mAb Em2G11. Furthermore, we detected spegs in the surrounding host tissue and in almost all tested lymph nodes (39/41) of infected patients. 38/47 lymph nodes of AE showed a positive reaction for spems with mAb EmG3, whereas 29/47 tested positive when stained with mAb Em2G11. Spegs were detected in the germinal centers, co-located with CD23-positive follicular dendritic cells, and were present in the sinuses. Likewise, lymph nodes with spems and spegs in AE and CE were significantly enlarged in size in comparison to the control group.Conclusions/significancemAb EmG3 is specific for AE and CE and is a valuable tool in the histological diagnosis of echinococcosis. Based on the observed staining patterns, we hypothesize that the interaction between parasite and host is not restricted to the main lesion since spegs are detected in lymph nodes. Moreover, in AE the number of spems-affected lymph nodes is higher than previously assumed. The enlargement of lymph nodes with spems and spegs points to an immunological interaction with the small immunogenic particles (spems and spegs) of Echinococcus spp.

Dataset Information

Sonography and sonoelastography in the detection of malignancy in superficial lymph nodes of dogs.

Publications

Sonography and sonoelastography in the detection of malignancy in superficial lymph nodes of dogs.

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