Dataset Information
MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1.
Ontology highlight
ABSTRACT: Background
While microRNAs (miRNAs) are known to play a critical role in the progression of colorectal cancer, the role of miR-107 remains unknown. We evaluated its role and explored the underlying mechanism.Materials & methods
MTT, wound-healing, transwell migration and transwell invasion assays were performed to evaluate the role of miR-107 in SW629 cell proliferation, migration and invasion. Real time-PCR and dual-luciferase reporter gene, TFR1 overexpression and western blotting assays were used to explore the underlying mechanism.Results
MiR-107 is downregulated in colorectal cancer tissues and several human colorectal cancer cell lines. Low miR-107 expression often indicates a poor survival rate for colorectal cancer patients. MiR-107 suppresses the proliferation, migration and invasion of SW620 cells by negatively regulating transferrin receptor 1 (TFR1).Conclusion
MiR-107 suppresses the metastasis of colorectal cancer and could be a potential therapy target in colorectal cancer patients.
SUBMITTER: Fu Y
PROVIDER: S-EPMC6524234 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1.
Cellular & molecular biology letters 20190516
<h4>Background</h4>While microRNAs (miRNAs) are known to play a critical role in the progression of colorectal cancer, the role of miR-107 remains unknown. We evaluated its role and explored the underlying mechanism.<h4>Materials & methods</h4>MTT, wound-healing, transwell migration and transwell invasion assays were performed to evaluate the role of miR-107 in SW629 cell proliferation, migration and invasion. Real time-PCR and dual-luciferase reporter gene, TFR1 overexpression and western blott ...[more]