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The VDR gene FokI polymorphism is associated with gestational diabetes mellitus in Turkish women.

ABSTRACT: BACKGROUND:The association between the vitamin D receptor (VDR) gene and gestational diabetes mellitus (GDM) has not been investigated in Turkish pregnant women. We aimed to investigate associations between VDR gene BsmI (rs15444410), ApaI (rs7975232), FokI (rs19735810), and TaqI (rs731236) single nucleotide polymorphisms (SNPs) and GDM. MATERIAL-METHODS:This case-control study comprised 100 women with GDM and 135 pregnant women without GDM. The VDR polymorphism was evaluated using Sanger-based DNA sequencing. RESULT:VDR gene ApaI, BsmI, and TaqI SNPs did not differ between women with and without GDM (each, p?>?0.05). ApaI, BsmI, and TaqI were not associated with GDM risk. The VDR gene FokI CT/TT genotype was associated with an increased GDM risk (CT vs. CC, OR?=?1.84, 95% CI: [1.05-3.23], p?=?0.031; TT vs. CC, OR?=?3.95, 95% CI: [1.56-9.96], p?=?0.002; CT/TT vs. CC, OR?=?2.29, 95% CI: [1.35-3.89], p?=?0.002; and CT/CC vs. TT, OR?=?3.02, 95% CI: [1.23-7.38], p?=?0.012). The FokI-TT genotype was more associated with younger age and higher glucose, HbA1c, and HOMA-IR than the CC and CT genotype. FokI-T was positively correlated with log-HOMA-IR (r?=?0.326, p?=?0.004). FokI SNPs were independently associated with GDM after adjusting for BMI and age (??=?1.63, 95% CI: [1. 2-4.2], p?=?0.012). There were no associations between the FokI, ApaI, BsmI and TaqI haplotypes and GDM. CONCLUSION:VDR gene FokI SNPs were independently associated with having GDM in Turkish women. VDR gene FokI SNPs might contribute to insulin resistance of developing GDM.

SUBMITTER: Apayd?n M

PROVIDER: S-EPMC6524275 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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The VDR gene FokI polymorphism is associated with gestational diabetes mellitus in Turkish women.

Apaydın Mahmut M Beysel Selvihan S Eyerci Nilnur N Pinarli Ferda Alparslan FA Ulubay Mustafa M Kizilgul Muhammed M Ozdemir Ozhan O Caliskan Mustafa M Cakal Erman E

BMC medical genetics 20190516 1

<h4>Background</h4>The association between the vitamin D receptor (VDR) gene and gestational diabetes mellitus (GDM) has not been investigated in Turkish pregnant women. We aimed to investigate associations between VDR gene BsmI (rs15444410), ApaI (rs7975232), FokI (rs19735810), and TaqI (rs731236) single nucleotide polymorphisms (SNPs) and GDM.<h4>Material-methods</h4>This case-control study comprised 100 women with GDM and 135 pregnant women without GDM. The VDR polymorphism was evaluated usin ...[more]

PMID: 31096931

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Project description:BACKGROUND AND AIMS:Genetic variants involved in vitamin D metabolism have been associated with diabetes and related syndromes/diseases. We wanted to investigate possible associations of polymorphisms in genes involved in vitamin D metabolism with indices of insulin resistance and insulin secretion, and also with development of diabetes after gestational diabetes mellitus (GDM). MATERIALS AND METHODS:We have studied 376 women with previous GDM. Eight single nucleotide polymorphisms (SNPs) in the genes for vitamin D receptor (VDR) [rs731236, rs7975232, rs10735810, and rs1544410], vitamin D binding protein (DBP) [rs7041 and rs4588], and cytochrome P450 family 27 subfamily B member 1 (CYP27B1) [rs10877012 and rs4646536] were genotyped by TaqMan Allelic Discrimination Assay using the Quantstudio 7 Flex system. A 75-g oral glucose tolerance test (OGTT) was performed 1-2 years postpartum. The homeostasis model assessment of insulin resistance (HOMA-IR) and the disposition index [(insulinogenic index: I30/G30)/HOMA-IR] were used to calculate insulin resistance and insulin secretion, respectively. Serum samples for determination of 25(OH)D3 were collected at the time of the OGTT. Manifestation of diabetes was followed up to five years postpartum. RESULTS:After adjustment for BMI, age, and ethnicity, the A-allele of the VDR rs1544410 polymorphism was found to be associated with increased disposition index (difference per allele = 3.56, 95% CI: 0.4567-6.674; p = 0.03). The A-allele of the DBP rs7041 polymorphism was found to be associated with 25(OH)D3 levels (difference [in nmol/L] per allele = -5.478, 95% CI: -8.315 to -2.641; p = 0.0002), as was the T-allele of the DBP rs4588 polymorphism (OR = -6.319, 95% CI: -9.466 to -3.171; p = 0.0001). None of the SNPs were significantly associated with HOMA-IR or postpartum diabetes. CONCLUSIONS:This study provides evidence that the rs1544410 polymorphism of the VDR gene may be associated with increased insulin secretion in women after pregnancy complicated by GDM. Further studies in other populations are needed to confirm the results.

Dataset Information

The VDR gene FokI polymorphism is associated with gestational diabetes mellitus in Turkish women.

Publications

The VDR gene FokI polymorphism is associated with gestational diabetes mellitus in Turkish women.

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