Isolation of novel gut bifidobacteria using a combination of metagenomic and cultivation approaches.
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ABSTRACT:
SUBMITTER: Lugli GA
PROVIDER: S-EPMC6524291 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Publications
Isolation of novel gut bifidobacteria using a combination of metagenomic and cultivation approaches.
Lugli Gabriele Andrea GA Milani Christian C Duranti Sabrina S Alessandri Giulia G Turroni Francesca F Mancabelli Leonardo L Tatoni Danilo D Ossiprandi Maria Cristina MC van Sinderen Douwe D Ventura Marco M
Genome biology 20190516 1
Whole metagenome shotgun (WMGS) sequencing is a method that provides insights into the genomic composition and arrangement of complex microbial consortia. Here, we report how WMGS coupled with a cultivation approach allows the isolation of novel bifidobacteria from animal fecal samples. A combination of in silico analyses based on nucleotide and protein sequences facilitate the identification of genetic material belonging to putative novel species. Consequently, the prediction of metabolic prope ...[more]
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