Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction.
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ABSTRACT:
SUBMITTER: Goto C
PROVIDER: S-EPMC6527390 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Publications
Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction.
Goto Chieko C Hashizume Shoko S Fukao Yoichiro Y Hara-Nishimura Ikuko I Tamura Kentaro K
Nucleus (Austin, Tex.) 20191201 1
In eukaryotes, the nucleus plays key roles in fundamental cellular processes, including DNA replication, chromatin maintenance, transcription, and translation. To better understand the functional diversity of nuclei, we developed a method for the comprehensive extraction of the nuclear proteome from Arabidopsis. We used a buffer with a high sucrose concentration to purify nuclei and then conducted solubility-based fractionation to increase proteome coverage. We identified 1539 proteins and two n ...[more]
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