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Characterization of biogenic selenium nanoparticles derived from cell-free extracts of a novel yeast Magnusiomyces ingens.

ABSTRACT: A facile one-pot and effective green process for biogenic selenium nanoparticles (SeNPs) was obtained using the cell-free extracts of a novel yeast Magnusiomyces ingens LH-F1. The corresponding absorption peak of SeNPs was observed at?~?560 nm by UV-vis spectrophotometer. In the present study, SeO2 2 mM, protein 500 mg L-1 and pH 7 were preferable to the biosynthesis of SeNPs. The effects of pH, SeO2 concentration and protein concentration on the synthesis process were different. Transmission electron microscopy image exhibited that all the SeNPs were spherical and quasi-spherical with the diameters mainly distributed in 70-90 nm (average particles size was 87.82?±?2.71 nm). X-ray diffraction suggested that the nanoparticles were composed of standard hexagonal crystalline Se with high purity. Fourier transform infrared spectroscopy indicated that some biomolecules such as hydroxyl, carboxyl and amino groups in the yeast cell-free extracts might be involved in the formation of SeNPs. Analyses of sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that two proteins with low molecular weight approximately?~?16 and?~?21 kDa were detected on the surface of SeNPs and in the extracts, which could play the role of natural stabilizers and confer stability to synthesized SeNPs; whereas, unbound proteins on the SeNPs surface could act as reducing agents. Antibacterial analysis showed that the SeNPs could inhibit Arthrobacter sp. W1 (Gram positive) but not E. coli BL21 (Gram negative), which could provide reference for antimicrobial application of biogenic SeNPs.

SUBMITTER: Lian S

PROVIDER: S-EPMC6527717 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Characterization of biogenic selenium nanoparticles derived from cell-free extracts of a novel yeast Magnusiomyces ingens.

Lian Shengyang S Diko Catherine Sekyerebea CS Yan Yongquan Y Li Zheng Z Zhang Henglin H Ma Qiao Q Qu Yuanyuan Y

3 Biotech 20190520 6

A facile one-pot and effective green process for biogenic selenium nanoparticles (SeNPs) was obtained using the cell-free extracts of a novel yeast Magnusiomyces ingens LH-F1. The corresponding absorption peak of SeNPs was observed at ~ 560 nm by UV-vis spectrophotometer. In the present study, SeO2 2 mM, protein 500 mg L-1 and pH 7 were preferable to the biosynthesis of SeNPs. The effects of pH, SeO2 concentration and protein concentration on the synthesis ...[more]

PMID: 31114745

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Project description:BackgroundAs antibiotics and chemotherapeutics are no longer as efficient as they once were, multidrug resistant (MDR) pathogens and cancer are presently considered as two of the most dangerous threats to human life. In this study, Selenium nanoparticles (SeNPs) biosynthesized by Streptomyces parvulus MAR4, nano-chitosan (NCh), and their nanoconjugate (Se/Ch-nanoconjugate) were suggested to be efficacious antimicrobial and anticancer agents.ResultsSeNPs biosynthesized by Streptomyces parvulus MAR4 and NCh were successfully achieved and conjugated. The biosynthesized SeNPs were spherical with a mean diameter of 94.2 nm and high stability. Yet, Se/Ch-nanoconjugate was semispherical with a 74.9 nm mean diameter and much higher stability. The SeNPs, NCh, and Se/Ch-nanoconjugate showed significant antimicrobial activity against various microbial pathogens with strong inhibitory effect on their tested metabolic key enzymes [phosphoglucose isomerase (PGI), pyruvate dehydrogenase (PDH), glucose-6-phosphate dehydrogenase (G6PDH) and nitrate reductase (NR)]; Se/Ch-nanoconjugate was the most powerful agent. Furthermore, SeNPs revealed strong cytotoxicity against HepG2 (IC50 = 13.04 μg/ml) and moderate toxicity against Caki-1 (HTB-46) tumor cell lines (IC50 = 21.35 μg/ml) but low cytotoxicity against WI-38 normal cell line (IC50 = 85.69 μg/ml). Nevertheless, Se/Ch-nanoconjugate displayed substantial cytotoxicity against HepG2 and Caki-1 (HTB-46) with IC50 values of 11.82 and 7.83 μg/ml, respectively. Consequently, Se/Ch-nanoconjugate may be more easily absorbed by both tumor cell lines. However, it exhibited very low cytotoxicity on WI-38 with IC50 of 153.3 μg/ml. Therefore, Se/Ch-nanoconjugate presented the most anticancer activity.ConclusionThe biosynthesized SeNPs and Se/Ch-nanoconjugate are convincingly recommended to be used in biomedical applications as versatile and potent antimicrobial and anticancer agents ensuring notable levels of biosafety, environmental compatibility, and efficacy.

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Characterization of biogenic selenium nanoparticles derived from cell-free extracts of a novel yeast Magnusiomyces ingens.

Publications

Characterization of biogenic selenium nanoparticles derived from cell-free extracts of a novel yeast <i>Magnusiomyces ingens</i>.

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