Association between blood microbiome and type 2 diabetes mellitus: A nested case-control study.

ABSTRACT:

Background

Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce.

Methods

Fifty incident T2DM cases and 100 matched non-T2DM controls were selected from a prospective cohort study of "135." The composition of the blood microbiome was characterized using bacterial 16S ribosomal RNA (16S rRNA) gene sequencing from pre-diagnostic blood sample. The amplicons were normalized, pooled, and sequenced on the Illumina MiSeq instrument using a MiSeq Reagent Kit PE300 v3 kit.

Results

Totally, 3 000 391 and 6 244 227 high-quality sequences were obtained from T2DM patients and non-T2DM controls, respectively. The me

SUBMITTER: Qiu J 

PROVIDER: S-EPMC6528574 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature