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Molecular characterization of peste-des-petits ruminants virus from Nepal, 2005 to 2016.


ABSTRACT: Outbreaks of peste-des-petits ruminants (PPR) has been reported regularly in Nepal since 1994. Despite this, there has been limited molecular characterization of circulating virus in the country. In this study a 351 bp segment of the nucleoprotein gene of the PPR virus (PPRV) was amplified and sequenced from ten samples collected between 2005 and 2016. Phylogenetic trees were estimated from these sequences using the maximum likelihood method confirming that all of the PPRV from the samples analysed belonged to the sub-clade IV of clade I of lineage IV and that they shared a common origin with other PPRV isolates in the region.

SUBMITTER: Pandey SK 

PROVIDER: S-EPMC6531496 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Molecular characterization of peste-des-petits ruminants virus from Nepal, 2005 to 2016.

Pandey Samjhana Kafle SK   Koirala Pragya P   Maharjan Manju M   Lamien Charles Euloge CE   Cattoli Giovanni G   Dundon William G WG   Settypalli Tirumala B K TBK  

Virusdisease 20190102 2


Outbreaks of peste-des-petits ruminants (PPR) has been reported regularly in Nepal since 1994. Despite this, there has been limited molecular characterization of circulating virus in the country. In this study a 351 bp segment of the nucleoprotein gene of the PPR virus (PPRV) was amplified and sequenced from ten samples collected between 2005 and 2016. Phylogenetic trees were estimated from these sequences using the maximum likelihood method confirming that all of the PPRV from the samples analy  ...[more]

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