Dynamic relocalization of replication origins by Fkh1 requires execution of DDK function and Cdc45 loading at origins.

ABSTRACT: Chromosomal DNA elements are organized into spatial domains within the eukaryotic nucleus. Sites undergoing DNA replication, high-level transcription, and repair of double-strand breaks coalesce into foci, although the significance and mechanisms giving rise to these dynamic structures are poorly understood. In S. cerevisiae, replication origins occupy characteristic subnuclear localizations that anticipate their initiation timing during S phase. Here, we link localization of replication origins in G1 phase with Fkh1 activity, which is required for their early replication timing. Using a Fkh1-dependent origin relocalization assay, we determine that execution of Dbf4-dependent kinase function, including Cdc45 loading, results in dynamic relocalization of a replication origin from the

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6533057 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature