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Sugar-induced de novo cytokinin biosynthesis contributes to Arabidopsis growth under elevated CO₂.

ABSTRACT: Carbon availability is a major regulatory factor in plant growth and development. Cytokinins, plant hormones that play important roles in various aspects of growth and development, have been implicated in the carbon-dependent regulation of plant growth; however, the details of their involvement remain to be elucidated. Here, we report that sugar-induced cytokinin biosynthesis plays a role in growth enhancement under elevated CO₂ in Arabidopsis thaliana. Growing Arabidopsis seedlings under elevated CO₂ resulted in an accumulation of cytokinin precursors that preceded growth enhancement. In roots, elevated CO₂ induced two genes involved in de novo cytokinin biosynthesis: an adenosine phosphate-isopentenyltransferase gene, AtIPT3, and a cytochrome P450 monooxygenase gene, CYP735A2. The expression of these genes was inhibited by a photosynthesis inhibitor, DCMU, under elevated CO₂, and was enhanced by sugar supplements, indicating that photosynthetically generated sugars are responsible for the induction. Consistently, cytokinin precursor accumulation was enhanced by sugar supplements. Cytokinin biosynthetic mutants were impaired in growth enhancement under elevated CO₂, demonstrating the involvement of de novo cytokinin biosynthesis for a robust growth response. We propose that plants employ a system to regulate growth in response to elevated CO₂ in which photosynthetically generated sugars induce de novo cytokinin biosynthesis for growth regulation.

SUBMITTER: Kiba T

PROVIDER: S-EPMC6533260 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Sugar-induced de novo cytokinin biosynthesis contributes to Arabidopsis growth under elevated CO<sub>2</sub>.

Kiba Takatoshi T Takebayashi Yumiko Y Kojima Mikiko M Sakakibara Hitoshi H

Scientific reports 20190523 1

Carbon availability is a major regulatory factor in plant growth and development. Cytokinins, plant hormones that play important roles in various aspects of growth and development, have been implicated in the carbon-dependent regulation of plant growth; however, the details of their involvement remain to be elucidated. Here, we report that sugar-induced cytokinin biosynthesis plays a role in growth enhancement under elevated CO<sub>2</sub> in Arabidopsis thaliana. Growing Arabidopsis seedlings u ...[more]

PMID: 31123308

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Project description:Global warming is becoming a significant problem for food security, particularly in the Mediterranean basin. The use of molecular techniques to study gene-level responses to environmental changes in non-model organisms is increasing and may help to improve the mechanistic understanding of durum wheat response to elevated CO2 and high temperature. With this purpose, we performed transcriptome RNA sequencing (RNA-Seq) analyses combined with physiological and biochemical studies in the flag leaf of plants grown in field chambers at ear emergence. Enhanced photosynthesis by elevated CO2 was accompanied by an increase in biomass and starch and fructan content, and a decrease in N compounds, as chlorophyll, soluble proteins, and Rubisco content, in association with a decline of nitrate reductase and initial and total Rubisco activities. While high temperature led to a decline of chlorophyll, Rubisco activity, and protein content, the glucose content increased and starch decreased. Furthermore, elevated CO2 induced several genes involved in mitochondrial electron transport, a few genes for photosynthesis and fructan synthesis, and most of the genes involved in secondary metabolism and gibberellin and jasmonate metabolism, whereas those related to light harvesting, N assimilation, and other hormone pathways were repressed. High temperature repressed genes for C, energy, N, lipid, secondary, and hormone metabolisms. Under the combined increases in atmospheric CO2 and temperature, the transcript profile resembled that previously reported for high temperature, although elevated CO2 partly alleviated the downregulation of primary and secondary metabolism genes. The results suggest that there was a reprogramming of primary and secondary metabolism under the future climatic scenario, leading to coordinated regulation of C-N metabolism towards C-rich metabolites at elevated CO2 and a shift away from C-rich secondary metabolites at high temperature. Several candidate genes differentially expressed were identified, including protein kinases, receptor kinases, and transcription factors.

Dataset Information

Sugar-induced de novo cytokinin biosynthesis contributes to Arabidopsis growth under elevated CO₂.

Publications

Sugar-induced de novo cytokinin biosynthesis contributes to Arabidopsis growth under elevated CO<sub>2</sub>.

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Dataset Information

Sugar-induced de novo cytokinin biosynthesis contributes to Arabidopsis growth under elevated CO2.

Publications

Sugar-induced de novo cytokinin biosynthesis contributes to Arabidopsis growth under elevated CO<sub>2</sub>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Sugar-induced de novo cytokinin biosynthesis contributes to Arabidopsis growth under elevated CO₂.