Ontology highlight
ABSTRACT:
SUBMITTER: Sonnett M
PROVIDER: S-EPMC6534117 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Sonnett Matthew M Gupta Meera M Nguyen Thao T Wühr Martin M
Methods in molecular biology (Clifton, N.J.) 20180101
The oocytes, embryos, and cell-free lysates of the frog Xenopus laevis have emerged as powerful models for quantitative proteomic experiments. In the accompanying paper (Chapter 13) we describe how to prepare samples and acquire multiplexed proteomics spectra from those. As an illustrative example we use a 10-stage developmental time series from the egg to stage 35 (just before hatching). Here, we outline how to convert the ~700,000 acquired mass spectra from this time series into protein expres ...[more]