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Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals.

ABSTRACT: Plants mainly acquire N from the soil in the form of nitrate (NO₃ ^-) or ammonium (NH₄ ⁺). Ammonium-based nutrition is gaining interest because it helps to avoid the environmental pollution associated with nitrate fertilization. However, in general, plants prefer NO₃ ^- and indeed, when growing only with NH₄ ⁺ they can encounter so-called ammonium stress. Since Brachypodium distachyon is a useful model species for the study of monocot physiology and genetics, we chose it to characterize performance under ammonium nutrition. Brachypodium distachyon Bd21 plants were grown hydroponically in 1 or 2.5 mM NO₃ ^- or NH₄ ⁺. Nitrogen and carbon metabolism associated with NH₄ ⁺ assimilation was evaluated in terms of tissue contents of NO₃ ^-, NH₄ ⁺, K, Mg, Ca, amino acids and organic acids together with tricarboxylic acid (TCA) cycle and NH₄ ⁺-assimilating enzyme activities and RNA transcript levels. The roots behaved as a physiological barrier preventing NH₄ ⁺ translocation to aerial parts, as indicated by a sizeable accumulation of NH₄ ⁺, Asn and Gln in the roots. A continuing high NH₄ ⁺ assimilation rate was made possible by a tuning of the TCA cycle and its associated anaplerotic pathways to match 2-oxoglutarate and oxaloacetate demand for Gln and Asn synthesis. These results show B. distachyon to be a highly suitable tool for the study of the physiological, molecular and genetic basis of ammonium nutrition in cereals.

SUBMITTER: de la Pena M

PROVIDER: S-EPMC6534281 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals.

de la Peña Marlon M González-Moro María Begoña MB Marino Daniel D

AoB PLANTS 20190510 3

Plants mainly acquire N from the soil in the form of nitrate (NO3 -) or ammonium (NH4 +). Ammonium-based nutrition is gaining interest because it helps to avoid the environmental pollution associated with nitrate fertilization. However, in general, plants prefer NO3 - and indeed, when growing only with NH4 + they can encounter so-called ammonium stress. Since Brachypodium distachyon is a useful model s ...[more]

PMID: 31139336

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Project description:BackgroundA complete assembled genome sequence of wheat is not yet available. Therefore, model plant systems for wheat are very valuable. Brachypodium distachyon (Brachypodium) is such a system. The WRKY family of transcription factors is one of the most important families of plant transcriptional regulators with members regulating important agronomic traits. Studies of WRKY transcription factors in Brachypodium and wheat therefore promise to lead to new strategies for wheat improvement.ResultsWe have identified and manually curated the WRKY transcription factor family from Brachypodium using a pipeline designed to identify all potential WRKY genes. 86 WRKY transcription factors were found, a total higher than all other current databases. We therefore propose that our numbering system (BdWRKY1-BdWRKY86) becomes the standard nomenclature. In the JGI v1.0 assembly of Brachypodium with the MIPS/JGI v1.0 annotation, nine of the transcription factors have no gene model and eleven gene models are probably incorrectly predicted. In total, twenty WRKY transcription factors (23.3%) do not appear to have accurate gene models. To facilitate use of our data, we have produced The Database of Brachypodium distachyon WRKY Transcription Factors. Each WRKY transcription factor has a gene page that includes predicted protein domains from MEME analyses. These conserved protein domains reflect possible input and output domains in signaling. The database also contains a BLAST search function where a large dataset of WRKY transcription factors, published genes, and an extensive set of wheat ESTs can be searched. We also produced a phylogram containing the WRKY transcription factor families from Brachypodium, rice, Arabidopsis, soybean, and Physcomitrella patens, together with published WRKY transcription factors from wheat. This phylogenetic tree provides evidence for orthologues, co-orthologues, and paralogues of Brachypodium WRKY transcription factors.ConclusionsThe description of the WRKY transcription factor family in Brachypodium that we report here provides a framework for functional genomics studies in an important model system. Our database is a resource for both Brachypodium and wheat studies and ultimately projects aimed at improving wheat through manipulation of WRKY transcription factors.

Dataset Information

Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals.

Publications

Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of <i>Brachypodium distachyon</i> for the study of ammonium stress in cereals.

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