Dataset Information

Soybean root transcriptome profiling reveals a nonhost resistant response during Heterodera glycines infection.

ABSTRACT: Heterodera glycines (soybean cyst nematode, SCN) is one of the most devastating pathogens of soybean worldwide. The compatible and in compatible interactions between soybean and SCN have well documented. Nevertheless, the molecular mechanism of a nonhost resistant response in soybean against SCN infection remains obscure. Toward this end, a global transcriptional comparison was conducted between susceptible and resistant reactions of soybean roots infected by taking advantage of finding a new pathotype of SCN (SCNT). The soybean cultivar Lee, which exhibits resistant to SCNT and susceptible to HG 1.2.3.4.7 (SCNs) was utilized in the expriments. The results highlighted a nonhost resistant response of soybean. Transcriptome analysis indicated that the number of differentially expressed genes (DEGs) in the resistant interaction (3746) was much larger than that in the susceptible interaction (602). A great number of genes acting as intrinsic component of membrane, integral component of membrane, cell periphery and plasma membrance were remarkably enriched only in the resistant interaction, while the taurine and hypotaurine, phenylpropanoid pathway, plant-pathogen interaction and transcript factors were modulated in both interactions. This is the first study to examine genes expression patterns in a soybean genotype in response to invasion by a virulent and avirulent SCN population at the transcriptional level, which will provide insights into the complicate molecular mechanism of the nonhost resistant interaction.

SUBMITTER: Song W

PROVIDER: S-EPMC6534303 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Soybean root transcriptome profiling reveals a nonhost resistant response during Heterodera glycines infection.

Song Wenwen W Qi Nawei N Liang Chen C Duan Fangmeng F Zhao Honghai H

PloS one 20190524 5

Heterodera glycines (soybean cyst nematode, SCN) is one of the most devastating pathogens of soybean worldwide. The compatible and in compatible interactions between soybean and SCN have well documented. Nevertheless, the molecular mechanism of a nonhost resistant response in soybean against SCN infection remains obscure. Toward this end, a global transcriptional comparison was conducted between susceptible and resistant reactions of soybean roots infected by taking advantage of finding a new pa ...[more]

PMID: 31125369

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Project description:The soybean cyst nematode (SCN), Heterodera glycines, causes economically significant damage to soybeans (Glycine max) in many parts of the world. The cysts of this nematode can remain quiescent in soils for many years as a reservoir of infection for future crops. To investigate bacterial communities associated with SCN cysts, cysts were obtained from eight SCN-infested farms in southern Ontario, Canada, and analyzed by culture-dependent and -independent means. Confocal laser scanning microscopy observations of cyst contents revealed a microbial flora located on the cyst exterior, within a polymer plug region and within the cyst. Microscopic counts using 5-(4,6-dichlorotriazine-2-yl)aminofluorescein staining and in situ hybridization (EUB 338) indicated that the cysts contained (2.6 +/- 0.5) x 10(5) bacteria (mean +/- standard deviation) with various cellular morphologies. Filamentous fungi were also observed. Live-dead staining indicated that the majority of cyst bacteria were viable. The probe Nile red also bound to the interior polymer, indicating that it is lipid rich in nature. Bacterial community profiles determined by denaturing gradient gel electrophoresis analysis were simple in composition. Bands shared by all eight samples included the actinobacterium genera Actinomadura and STREPTOMYCES: A collection of 290 bacteria were obtained by plating macerated surface-sterilized cysts onto nutrient broth yeast extract agar or on actinomycete medium. These were clustered into groups of siblings by repetitive extragenic palindromic PCR fingerprinting, and representative isolates were tentatively identified on the basis of 16S rRNA gene sequence. Thirty phylotypes were detected, with the collection dominated by Lysobacter and Variovorax spp. This study has revealed the cysts of this important plant pathogen to be rich in a variety of bacteria, some of which could presumably play a role in the ecology of SCN or have potential as biocontrol agents.

Project description:Soybean is an important economic crop that is often adversely affected by infection in the field with the soybean cyst nematode Heterodera glycines. Biological control is an eco-friendly method used to protect the crop against disease. The bacterium Klebsiella pneumoniae has been reported to protect rice from sheath blight and seedling blight, but its role in the control of nematode is unclear. In this study, the effect of K. pneumoniae SnebYK on the control of H. glycines was assessed. Potting experiment results showed that coating soybean seeds with K. pneumoniae SnebYK not only reduced the infection rate of H. glycines but also decreased the proportion of adult female nematodes. Field experiment results showed that K. pneumoniae SnebYK reduced both the number of H. glycines in soybean roots and the number of adult females. However, K. pneumoniae SnebYK caused low juvenile mortality in an in vitro assay. To further analyze the role of K. pneumoniae SnebYK in the inhibition of H. glycines infection, split root experiments were conducted. The results indicated that K. pneumoniae SnebYK controls H. glycines via induced systemic resistance, which reduces H. glycines penetration. Klebsiella pneumoniae SnebYK treatment also significantly increased the proportion of second-stage juveniles and decreased the proportions of third- and fourth-stage juveniles in the H. glycines population. Moreover, 48 h after inoculation with H. glycines, the expression levels of PR1, PR2, PR5, and PDF1.2 were significantly higher in soybeans pretreated with K. pneumoniae SnebYK than in control soybeans. Interestingly, besides providing protection against nematodes, K. pneumoniae SnebYK fixed nitrogen, produced ammonia, solubilized phosphate, and produced siderophores, leading to well-developed root system and an increase in soybean seedling fresh weight. These results demonstrate for the first time that K. pneumoniae SnebYK not only promotes soybean growth but also inhibits the invasion and development of H. glycines by inducing systemic resistance.

Dataset Information

Soybean root transcriptome profiling reveals a nonhost resistant response during Heterodera glycines infection.

Publications

Soybean root transcriptome profiling reveals a nonhost resistant response during Heterodera glycines infection.

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