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EFFECTS OF EXERCISE ON MIR-29 AND IGF-1 EXPRESSION AND LIPID PROFILE IN THE HEART OF OVARIECTOMIZED RAT.

ABSTRACT: Introduction:Menopause increases the risk of cardiovascular disease in women. The aims of the present study were to evaluate the effects of swimming training on cardiac histology and expression of miR-29 and IGF-1 in the ovariectomized rats. Materials and methods:Thirty female Wistar rats were divided into sham and ovariectomized groups: sedentary control (OVX) and trained with 8 weeks exercise (OVX.E). On 57th day, blood was collected and used for lipid profile measurement. In addition, heart tissue was analyzed by reverse transcription-polymerase chain reaction for IGF-1 mRNA and miR-29, and studied for histopathological changes. Results:Ovariectomy significantly decreased miR-29 and IGF-1 expression in the heart compared to sham animals group (p<0.05). Exercise training increased miR-29 and IGF-1 expression in the trained rats and improved histology and lipid profile compared with OVX group (p<0.05). Conclusion:Estrogen deficiency could lead to cardiac fibrosis through deregulation miR-29 and IGF-1 expression. The findings of the current study suggests a protective effect of exercise on heart against fibrotic changes in ovariectomized rats and support a potential preventive value of exercise in improving cardiac function after menopause.

SUBMITTER: Habibi P

PROVIDER: S-EPMC6535292 | biostudies-literature | 2016 Apr-Jun

REPOSITORIES: biostudies-literature

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EFFECTS OF EXERCISE ON MIR-29 AND IGF-1 EXPRESSION AND LIPID PROFILE IN THE HEART OF OVARIECTOMIZED RAT.

Habibi P P Alihemmatti A A Alipour M M Nourazar A A Yousefi H H Andalib S S Ahmadiasl N N

Acta endocrinologica (Bucharest, Romania : 2005) 20160401 2

<h4>Introduction</h4>Menopause increases the risk of cardiovascular disease in women. The aims of the present study were to evaluate the effects of swimming training on cardiac histology and expression of miR-29 and IGF-1 in the ovariectomized rats.<h4>Materials and methods</h4>Thirty female Wistar rats were divided into sham and ovariectomized groups: sedentary control (OVX) and trained with 8 weeks exercise (OVX.E). On 57<sup>th</sup> day, blood was collected and used for lipid profile measure ...[more]

PMID: 31149077

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Project description:Background:The prevalence of nonalcoholic steatohepatitis (NASH) in menopausal women is increasing, but current treatments have not been proven effective. The objective of this study was to investigate the treatment effects of genistein and running exercise in ovariectomized (OVX) rats with NASH.Methods:Thirty-six female Sprague-Dawley rats were divided into 6 groups, control; OVX with standard diet; OVX with high fat and high fructose (HFHF) diet for 4?weeks; OVX with HFHF and genistein treatment (16?mg/kg BW/day) for 5?weeks (OVX?+?HFHF+GEN); OVX with HFHF and moderate intensity exercise for 5?weeks (OVX?+?HFHF+EX); OVX with HFHF and combined treatments (OVX?+?HFHF+GEN?+?EX). Serum interleukin-6 (IL-6) levels, hepatic free fatty acid (FFA), hepatic glutathione (GSH), and hepatic malondialdehyde (MDA) levels were measured. Liver histology was examined to determine NASH severity.Results:OVX?+?HFHF group had the highest levels of hepatic FFA compared with OVX and control groups (5.92?±?0.84 vs. 0.37?±?0.01 vs. 0.42?±?0.04?nmol/mg protein, respectively, p?<?0.01). Serum IL-6 levels were significantly elevated in both OVX and OVX?+?HFHF groups as compared with controls (112.13?±?6.50 vs. 121.47?±?3.96 vs. 86.13?±?2.40?pg/mL, respectively, p?<?0.01). In OVX?+?HFHF group, hepatic MDA levels were higher, while GSH levels were lower than in OVX and control groups (MDA; 0.98?±?0.04 vs. 0.82?±?0.02 vs. 0.78?±?0.03?nmol/mg protein, and GSH; 46.01?±?0.91 vs. 55.21?±?1.40 vs. 57.94?±?0.32, respectively; p?<?0.01 for both). Comparing with OVX?+?HFHF group, rats that received genistein, exercise and combined treatments demonstrated an improvement in liver histopathology, decreased levels of hepatic FFA (1.44?±?0.21 vs. 0.45?±?0.04 vs. 0.49?±?0.05?nmol/mg protein, respectively, p?<?0.01), serum IL-6 (82.80?±?2.07 vs. 83.47?±?2.81 vs. 94.13?±?1.61?pg/mL, respectively, p?<?0.01), and hepatic MDA (0.80?±?0.03 vs. 0.76?±?0.02 vs. 0.76?±?0.03?nmol/mg protein, respectively, p?<?0.01).Conclusions:Genistein and moderate intensity exercise were effective in reducing the severity of NASH in OVX rats through the reduction in liver inflammation, oxidative stress and liver fat contents.

Project description:AimsIn post-menopausal women, incidence of heart failure with preserved ejection fraction is higher than in men. Hormonal replacement therapies did not demonstrate benefits. We tested whether the non-steroidal mineralocorticoid receptor antagonist finerenone limits the progression of heart failure in ovariectomized (OVX) mice with metabolic disorders.Methods and resultsOvariectomy was performed in 4-month-old mice, treated or not at 7 months old for 1 month with finerenone (Fine) 1 mg/kg/day. Left ventricular (LV) cardiac and coronary endothelial functions were assessed by echocardiography, catheterization, and myography. Blood pressure was measured by plethysmography. Insulin and glucose tolerance tests were performed. Exercise capacity and spontaneous activity were measured on treadmill and in combined indirect calorimetric cages equipped with voluntary running wheel. OVX mice presented LV diastolic dysfunction without modification of ejection fraction compared with controls (CTL), whereas finerenone improved LV filling pressure (LV end-diastolic pressure, mmHg: CTL 3.48 ± 0.41, OVX 6.17 ± 0.30**, OVX + Fine 3.65 ± 0.55† , **P < 0.01 vs. CTL, † P < 0.05 vs. OVX) and compliance (LV end-diastolic pressure-volume relation, mmHg/RVU: CTL 1.65 ± 0.42, OVX 4.77 ± 0.37***, OVX + Fine 2.87 ± 0.26†† , ***P < 0.001 vs. CTL, †† P < 0.01 vs. OVX). Acetylcholine-induced endothelial-dependent relaxation of coronary arteries was impaired in ovariectomized mice and improved by finerenone (relaxation, %: CTL 86 ± 8, OVX 38 ± 3**, OVX + Fine 83 ± 7†† , **P < 0.01 vs. CTL, †† P < 0.01 vs. OVX). Finerenone improved decreased ATP production by subsarcolemmal mitochondria after ovariectomy. Weight gain, increased blood pressure, and decreased insulin and glucose tolerance in OVX mice were improved by finerenone. The exercise capacity at race was diminished in untreated OVX mice only. Spontaneous activity measurements in ovariectomized mice showed decreased horizontal movements, reduced time spent in a running wheel, and reduced VO2 and VCO2 , all parameters improved by finerenone.ConclusionsFinerenone improved cardiovascular dysfunction and exercise capacity after ovariectomy-induced LV diastolic dysfunction with preserved ejection fraction.

Dataset Information

EFFECTS OF EXERCISE ON MIR-29 AND IGF-1 EXPRESSION AND LIPID PROFILE IN THE HEART OF OVARIECTOMIZED RAT.

Publications

EFFECTS OF EXERCISE ON MIR-29 AND IGF-1 EXPRESSION AND LIPID PROFILE IN THE HEART OF OVARIECTOMIZED RAT.

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