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Evaluation of HCP5 and Chemokine C Receptor type 5 Gene Polymorphisms in Indian Psoriatic Patients.

ABSTRACT: Background:Genetic variations associated with nonprogression of HIV infection to AIDS are enriched in psoriasis patients. HCP5 gene 335 T > G and chemokine C receptor type 5 (CCR5) gene ?32 polymorphisms are associated with HIV nonprogression phenotype. Aim:The aim of this study was to determine the association of HCP5 gene 335 T > G (rs2395029) and CCR5 gene ?32 (rs333) polymorphisms with psoriasis vulgaris (PV). Materials and Methods:Genotype of HCP5 gene 335 T > G and CCR5 gene ?32 polymorphisms were determined by polymerase chain reaction (PCR)-restriction fragment length polymorphism and allele-specific PCR methods, respectively. Results:The frequency of HCP5 gene 335 T > G SNP was ~7 times higher in PV patients than in the control group (P = 1.49 × 10-8; odds ratio [OR] = 10.2; 0.95 confidence interval [CI]: 3.9-26.8). OR for the occurrence of HCP5 335 G allele in either homozygous or heterozygous genotype in PV patients was 13.1 (0.95 CI: 4.7-36.1). The strength of association was higher with moderate-to-severe subgroup (P = 3.29 × 10-9; OR = 18.4; 0.95 CI: 6.2-54.9) than with mild subgroup (P = 2.1 × 10-4; OR = 8.3; 0.95 CI: 2.6-23.3). In addition, the strength of association was higher with Type I (P = 9.53 × 10-8; OR = 15.3; 0.95 CI: 5.1-46.5) than with Type II subgroup (P = 6.8 × 10-6; OR = 11.0; 0.95 CI: 3.6-33.9). Type I gene ?32 polymorphism was observed neither among psoriatic nor among healthy individuals. Conclusions:Our results indicate that HCP5 gene 335 T > G polymorphism and not CCR5 gene ?32 polymorphism is associated with the increased risk of developing PV.

SUBMITTER: Rajesh D

PROVIDER: S-EPMC6537696 | biostudies-literature | 2019 May-Jun

REPOSITORIES: biostudies-literature

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Evaluation of <i>HCP5</i> and <i>Chemokine C Receptor type</i> 5 Gene Polymorphisms in Indian Psoriatic Patients.

Rajesh Deepa D Nagraj Sudeep S Kumar K S Praveen KSP Kutty A V Moideen AVM Balakrishna Sharath S

Indian journal of dermatology 20190501 3

<h4>Background</h4>Genetic variations associated with nonprogression of HIV infection to AIDS are enriched in psoriasis patients. <i>HCP5</i> gene 335 T > G and <i>chemokine C receptor type 5 (CCR5)</i> gene Δ32 polymorphisms are associated with HIV nonprogression phenotype.<h4>Aim</h4>The aim of this study was to determine the association of <i>HCP5</i> gene 335 T > G (rs2395029) and <i>CCR5</i> gene Δ32 (rs333) polymorphisms with psoriasis vulgaris (PV).<h4>Materials and methods</h4>Genotype o ...[more]

PMID: 31148855

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Project description:BACKGROUND: Diabetic retinopathy (DR) is classically defined as a microvasculopathy that primarily affects the small blood vessels of the inner retina as a complication of diabetes mellitus (DM).It is a multifactorial disease with a strong genetic component. The aim of this study is to investigate the association of a set of nine candidate genes with the development of diabetic retinopathy in a South Indian cohort who have type 2 diabetes mellitus (T2DM). METHODS: Seven candidate genes (RAGE, PEDF, AKR1B1, EPO, HTRA1, ICAM and HFE) were chosen based on reported association with DR in the literature. Two more, CFH and ARMS2, were chosen based on their roles in biological pathways previously implicated in DR. Fourteen single nucleotide polymorphisms (SNPs) and one dinucleotide repeat polymorphism, previously reported to show association with DR or other related diseases, were genotyped in 345 DR and 356 diabetic patients without retinopathy (DNR). The genes which showed positive association in this screening set were tested further in additional sets of 100 DR and 90 DNR additional patients from the Aravind Eye Hospital. Those which showed association in the secondary screen were subjected to a combined analysis with the 100 DR and 100 DNR subjects previously recruited and genotyped through the Sankara Nethralaya Hospital, India. Genotypes were evaluated using a combination of direct sequencing, TaqMan SNP genotyping, RFLP analysis, and SNaPshot PCR assays. Chi-square and Fisher exact tests were used to analyze the genotype and allele frequencies. RESULTS: Among the nine loci (15 polymorphisms) screened, SNP rs2070600 (G82S) in the RAGE gene, showed significant association with DR (allelic P = 0.016, dominant model P = 0.012), compared to DNR. SNP rs2070600 further showed significant association with DR in the confirmation cohort (P = 0.035, dominant model P = 0.032). Combining the two cohorts gave an allelic P < 0.003 and dominant P = 0.0013). Combined analysis with the Sankara Nethralaya cohort gave an allelic P = 0.0003 and dominant P = 0.00011 with an OR = 0.49 (0.34 - 0.70) for the minor allele. In HTRA1, rs11200638 (G>A), showed marginal significance with DR (P = 0.055) while rs10490924 in LOC387715 gave a P = 0.07. No statistical significance was observed for SNPs in the other 7 genes studied. CONCLUSIONS: This study confirms significant association of one polymorphism only (rs2070600 in RAGE) with DR in an Indian population which had T2DM.

Dataset Information

Evaluation of HCP5 and Chemokine C Receptor type 5 Gene Polymorphisms in Indian Psoriatic Patients.

Publications

Evaluation of <i>HCP5</i> and <i>Chemokine C Receptor type</i> 5 Gene Polymorphisms in Indian Psoriatic Patients.

Similar Datasets

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