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Simple alkanoyl acylating agents for reversible RNA functionalization and control.

ABSTRACT: We describe the synthesis and RNA acylation activity of a series of minimalist azidoalkanoyl imidazole reagents, with the aim of functionalizing RNA at 2'-hydroxyl groups at stoichiometric to superstoichiometric levels. We find marked effects of small structural changes on their ability to acylate and be reductively removed, and identify reagents and methods that enable efficient RNA functionalization and control.

SUBMITTER: Park HS

PROVIDER: S-EPMC6541391 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Simple alkanoyl acylating agents for reversible RNA functionalization and control.

Park Hyun Shin HS Kietrys Anna M AM Kool Eric T ET

Chemical communications (Cambridge, England) 20190401 35

We describe the synthesis and RNA acylation activity of a series of minimalist azidoalkanoyl imidazole reagents, with the aim of functionalizing RNA at 2'-hydroxyl groups at stoichiometric to superstoichiometric levels. We find marked effects of small structural changes on their ability to acylate and be reductively removed, and identify reagents and methods that enable efficient RNA functionalization and control. ...[more]

PMID: 30977472

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