The Effect of Influenza Virus on the Human Oropharyngeal Microbiome.

ABSTRACT:

Background

Secondary bacterial infections are an important cause of morbidity and mortality associated with influenza infections. As bacterial disease can be caused by a disturbance of the host microbiome, we examined the impact of influenza on the upper respiratory tract microbiome in a human challenge study.

Methods

The dynamics and ecology of the throat microbiome were examined following an experimental influenza challenge of 52 previously-healthy adult volunteers with influenza A/Wisconsin/67/2005 (H3N2) by intranasal inoculation; 35 healthy control subjects were not subjected to the viral challenge. Serial oropharyngeal samples were taken over a 30-day period, and the V1-V3 region of the bacterial 16S ribosomal RNA sequences were amplified and sequenced to determine the

SUBMITTER: Ramos-Sevillano E 

PROVIDER: S-EPMC6541733 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature