Ontology highlight
ABSTRACT:
SUBMITTER: Bao Z
PROVIDER: S-EPMC6541955 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Bao Zhenzhen Z Huang Yanli Y Chen Jiyu J Wang Zhenglong Z Qian Jiang J Xu Jiyang J Zhao Yucheng Y
BioMed research international 20190516
RAW264.7 is a macrophage strain derived from mice tumour and shows a significant ability in antigen uptake. Real-time quantitative PCR (RT-qPCR) is one of the most commonly used methods in gene studies and requires suitable reference genes to normalize and quantitate the expression of gene of interest with sensitivity and specificity. However, suitable reference genes in RAW264.7 cells have not yet been identified for accurate gene expression quantification. In the current study, we evaluated ex ...[more]