Methodological Issues in Studying PAHSA Biology: Masking PAHSA Effects.
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ABSTRACT:
SUBMITTER: Syed I
PROVIDER: S-EPMC6542592 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Publications
Methodological Issues in Studying PAHSA Biology: Masking PAHSA Effects.
Syed Ismail I Lee Jennifer J Peroni Odile D OD Yore Mark M MM Moraes-Vieira Pedro M PM Santoro Anna A Wellenstein Kerry K Smith Ulf U McGraw Timothy E TE Saghatelian Alan A Kahn Barbara B BB
Cell metabolism 20180920 4
PAHSAs are anti-diabetic and anti-inflammatory lipids. Syed et al. identify numerous experimental differences that likely account for the failure of Pflimlin et al. to observe PAHSA beneficial effects. The differences include different HFDs resulting in minimal/no glucose intolerance, different assay conditions, an LC-MS protocol that was not validated, and use of olive oil, a bioactive nutrient that improves glucose tolerance, as a vehicle. ...[more]
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