Dataset Information

Deficiency in Silicon Transporter Lsi1 Compromises Inducibility of Anti-herbivore Defense in Rice Plants.

ABSTRACT: Silicon (Si) application can significantly enhance rice resistance against herbivorous insects. However, the underlying mechanism is elusive. In this study, silicon transporter mutant OsLsi1 and corresponding wild-type rice (WT) were treated with and without Si to determine Si effects on rice resistance to leaffolder (LF), Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Pyralidae). Si application on WT plants significantly promoted rice plant growth, upregulated expression level of OsLsi1 and increased Si accumulation in the leaves and roots, as well as effectively reduced LF weight gain, while it showed only marginal or no effect on the mutant plants. Furthermore, upon LF infestation, transcript levels of OsLOX, OsAOS2, OsCOI1a, OsCOI1b, and OsBBPI, and activity of catalase, superoxide dismutase, peroxidase, and polyphenol oxidase were significantly higher in Si-treated than untreated WT plants. However, OsLsi1 mutant plants displayed higher susceptibility to LF, and minimal response of defense-related enzymes and jasmonate dependent genes to Si application. These results suggest that induced defense plays a vital role in Si-enhanced resistance and deficiency in silicon transporter Lsi1 compromises inducibility of anti-herbivore defense in rice plants.

SUBMITTER: Lin Y

PROVIDER: S-EPMC6543919 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Deficiency in Silicon Transporter Lsi1 Compromises Inducibility of Anti-herbivore Defense in Rice Plants.

Lin Yibin Y Sun Zhongxiang Z Li Zhenfang Z Xue Rongrong R Cui Weikang W Sun Shaozhi S Liu Tingting T Zeng Rensen R Song Yuanyuan Y

Frontiers in plant science 20190524

Silicon (Si) application can significantly enhance rice resistance against herbivorous insects. However, the underlying mechanism is elusive. In this study, silicon transporter mutant <i>OsLsi1</i> and corresponding wild-type rice (WT) were treated with and without Si to determine Si effects on rice resistance to leaffolder (LF), <i>Cnaphalocrocis medinalis</i> (Guenée) (Lepidoptera: Pyralidae). Si application on WT plants significantly promoted rice plant growth, upregulated expression level of ...[more]

PMID: 31178878

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Project description:Silicon-based defenses deter insect herbivores in many cultivated and wild grass species. Furthermore, in some of these species, silicon (Si) uptake and defense can be induced by herbivory. Tropical trees also take up Si and leaf Si concentrations vary greatly across and within species. As herbivory is a major driver of seedling mortality and niche differentiation of tropical tree species, understanding anti-herbivore defenses is pivotal. Yet, whether silicon is a constitutive and inducible herbivory defense in tropical forest tree species remains unknown. We grew seedlings of eight tropical tree species in a full factorial experiment, including two levels of plant-available soil Si concentrations (-Si/+Si) and a simulated herbivory treatment (-H/+H). The simulated herbivory treatment was a combination of clipping and application of methyl jasmonate. We then carried out multiple-choice feeding trials, separately for each tree species, in which leaves of each treatment combination were offered to a generalist caterpillar (Spodoptera frugiperda). Leaf damage was assessed. Three species showed a significant decrease in leaf damage under high compared to low Si conditions (by up to 72%), consistent with our expectation of Si-based defenses acting in tropical tree species. In one species, leaf damage was increased by increasing soil Si and in four species, no effect of soil Si on leaf damage was observed. Opposite to our expectation of Si uptake and defense being inducible by herbivory damage, simulated herbivory increased leaf damage in two species. Furthermore, simulated herbivory reduced Si concentrations in one species. Our results showed that tropical tree seedlings can be better defended when growing in Si-rich compared to Si-poor soils, and that the effects of Si on plant defense vary strongly across species. Furthermore, Si-based defenses may not be inducible in tropical tree species. Overall, constitutive Si-based defense should be considered part of the vast array of anti-herbivore defenses of tropical tree species. Our finding that Si-based defenses are highly species-specific combined with the fact that herbivory is a major driver of mortality in tropical tree seedling, suggests that variation in soil Si concentrations may have pervasive consequences for regeneration and performance across tropical tree species.

Dataset Information

Deficiency in Silicon Transporter Lsi1 Compromises Inducibility of Anti-herbivore Defense in Rice Plants.

Publications

Deficiency in Silicon Transporter Lsi1 Compromises Inducibility of Anti-herbivore Defense in Rice Plants.

Similar Datasets

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