Ontology highlight
ABSTRACT:
SUBMITTER: Kishi JY
PROVIDER: S-EPMC6544483 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Kishi Jocelyn Y JY Lapan Sylvain W SW Beliveau Brian J BJ West Emma R ER Zhu Allen A Sasaki Hiroshi M HM Saka Sinem K SK Wang Yu Y Cepko Constance L CL Yin Peng P
Nature methods 20190520 6
Fluorescence in situ hybridization (FISH) reveals the abundance and positioning of nucleic acid sequences in fixed samples. Despite recent advances in multiplexed amplification of FISH signals, it remains challenging to achieve high levels of simultaneous amplification and sequential detection with high sampling efficiency and simple workflows. Here we introduce signal amplification by exchange reaction (SABER), which endows oligonucleotide-based FISH probes with long, single-stranded DNA concat ...[more]