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Self-Assembled DNA Tubes Forming Helices of Controlled Diameter and Chirality.

ABSTRACT: Multihelical DNA bundles could enhance the functionality of nanomaterials and serve as model architectures to mimic protein filaments on the molecular and cellular level. We report the self-assembly of micrometer-sized helical DNA nanotubes with widely controllable helical diameters ranging from tens of nanometers to a few micrometers. Nanoscale helical shapes of DNA tile tubes (4-, 6-, 8-, 10-, and 12-helix tile tubes) are achieved by introducing discrete amounts of bending and twist through base pair insertions and/or deletions. Microscale helical diameters, which require smaller amounts of twist and bending, are achieved by controlling the intrinsic "supertwist" present in tile tubes with uneven number of helices (11-, 13-, and 15-helix tile tubes). Supertwist fine-tuning also allows us to produce helical nanotubes of defined chirality.

SUBMITTER: Maier AM

PROVIDER: S-EPMC6544521 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Self-Assembled DNA Tubes Forming Helices of Controlled Diameter and Chirality.

Maier Alexander Mario AM Bae Wooli W Schiffels Daniel D Emmerig Johannes Friedrich JF Schiff Maximilian M Liedl Tim T

ACS nano 20170131 2

Multihelical DNA bundles could enhance the functionality of nanomaterials and serve as model architectures to mimic protein filaments on the molecular and cellular level. We report the self-assembly of micrometer-sized helical DNA nanotubes with widely controllable helical diameters ranging from tens of nanometers to a few micrometers. Nanoscale helical shapes of DNA tile tubes (4-, 6-, 8-, 10-, and 12-helix tile tubes) are achieved by introducing discrete amounts of bending and twist through ba ...[more]

PMID: 28125777

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