Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model.
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ABSTRACT:
SUBMITTER: Virdi V
PROVIDER: S-EPMC6544532 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
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Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model.
Virdi Vikram V Palaci Jorge J Laukens Bram B Ryckaert Stefan S Cox Eric E Vanderbeke Erik E Depicker Ann A Callewaert Nico N
Nature biotechnology 20190401 5
Oral antibodies that interfere with gastrointestinal targets and can be manufactured at scale are needed. Here we show that a single-gene-encoded monomeric immunoglobulin A (IgA)-like antibody, composed of camelid variable single domain antibodies (VHH) fused to IgA Fc (mVHH-IgA), prevents infection by enterotoxigenic Escherichia coli (F4-ETEC) in piglets. The mVHH-IgA can be produced in soybean seeds or secreted from the yeast Pichia pastoris, freeze- or spray-dried and orally delivered within ...[more]
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