Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference.
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ABSTRACT:
SUBMITTER: Xiong K
PROVIDER: S-EPMC6545153 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Publications
Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference.
Xiong Kai K Marquart Kim Fabiano KF la Cour Karottki Karen Julie KJ Li Shangzhong S Shamie Isaac I Lee Jae Seong JS Gerling Signe S Yeo Nan Cher NC Chavez Alejandro A Lee Gyun Min GM Lewis Nathan E NE Kildegaard Helene Faustrup HF
Biotechnology and bioengineering 20190402 7
Chinese hamster ovary (CHO) cells are widely used for biopharmaceutical protein production. One challenge limiting CHO cell productivity is apoptosis stemming from cellular stress during protein production. Here we applied CRISPR interference (CRISPRi) to downregulate the endogenous expression of apoptotic genes Bak, Bax, and Casp3 in CHO cells. In addition to reduced apoptosis, mitochondrial membrane integrity was improved and the caspase activity was reduced. Moreover, we optimized the CRISPRi ...[more]
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