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Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration.

ABSTRACT: Poly(L-lactide-co-?-caprolactone) scaffolds were functionalised by 10 or 20?µg/mL of human demineralised dentine matrix. Release kinetics up to 21?days and their osteogenic potential on human bone marrow stromal cells after 7 and 21?days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20?µg/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7?days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21?days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21?days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.

SUBMITTER: Munir A

PROVIDER: S-EPMC6545639 | biostudies-literature | 2019 Jan-Dec

REPOSITORIES: biostudies-literature

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Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration.

Munir Arooj A Døskeland Anne A Avery Steven J SJ Fuoco Tiziana T Mohamed-Ahmed Samih S Lygre Henning H Finne-Wistrand Anna A Sloan Alastair J AJ Waddington Rachel J RJ Mustafa Kamal K Suliman Salwa S

Journal of tissue engineering 20190101

Poly(L-lactide-co-ε-caprolactone) scaffolds were functionalised by 10 or 20 µg/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 µg/mL and cultured in basal medium (DDM group) ...[more]

PMID: 31210921

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Project description:Here we present a new model for alveolar jaw bone regeneration, which uses human dental pulp cells (hDPCs) combined with tyrosine-derived polycarbonate polymer scaffolds [E1001(1k)] containing beta-tricalcium phosphate (?-TCP) [E1001(1k)/?-TCP]. E1001(1k)/?-TCP scaffolds (5?mm diameter?×?1?mm thickness) were fabricated to fit a 5?mm rat mandibular ramus critical bone defect. Five experimental groups were examined in this study: (1) E1001(1k)/?-TCP scaffolds seeded with a high density of hDPCs, 5.0?×?10(5) hDPCs/scaffold (CH); (2) E1001(1k)/?-TCP scaffolds seeded with a lower density of hDPCs, 2.5?×?10(5) hDPCs/scaffold (CL); (3) acellular E1001(1k)/?-TCP scaffolds (SA); (4) acellular E1001(1k)/?-TCP scaffolds supplemented with 4??g recombinant human bone morphogenetic protein-2 (BMP); and (5) empty defects (EDs). Replicate hDPC-seeded and acellular E1001(1k)/?-TCP scaffolds were cultured in vitro in osteogenic media for 1 week before implantation for 3 and 6 weeks. Live microcomputed tomography (?CT) imaging at 3 and 6 weeks postimplantation revealed robust bone regeneration in the BMP implant group. CH and CL groups exhibited similar uniformly distributed mineralized tissue coverage throughout the defects, but less than the BMP implants. In contrast, SA-treated defects exhibited sparse areas of mineralized tissue regeneration. The ED group exhibited slightly reduced defect size. Histological analyses revealed no indication of an immune response. In addition, robust expression of dentin and bone differentiation marker expression was observed in hDPC-seeded scaffolds, whereas, in contrast, BMP and SA implants exhibited only bone and not dentin differentiation marker expression. hDPCs were detected in 3-week but not in 6-week hDPC-seeded scaffold groups, indicating their survival for at least 3 weeks. Together, these results show that hDPC-seeded E1001(1k)/?-TCP scaffolds support the rapid regeneration of osteo-dentin-like mineralized jaw tissue, suggesting a promising new therapy for alveolar jaw bone repair and regeneration.

Dataset Information

Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration.

Publications

Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration.

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