Optimizing DNA transduction by selection of mutations that evade bacterial defense systems.
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ABSTRACT:
SUBMITTER: Auster O
PROVIDER: S-EPMC6546373 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Publications
Optimizing DNA transduction by selection of mutations that evade bacterial defense systems.
Auster Oren O Globus Rea R Yosef Ido I Qimron Udi U
RNA biology 20180913 4
We recently developed a platform where phage-transducing particles optimize DNA delivery to a wide range of hosts. Here, we use this platform to optimize DNA transduction into hosts that naturally restrict specific DNA sequences. We first show that a specific plasmid is restricted for transduction into a particular Salmonella strain. Using the platform, we select for a mutated plasmid that overcomes the restriction barrier. Insertion of the non-mutated sequence into a permissive plasmid restrict ...[more]
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