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Identification of Hfq-binding RNAs in Caulobacter crescentus.

ABSTRACT: Small RNAs are important for post-transcriptional regulation of gene expression, affecting stability and activity of their target mRNAs. The bacterial Sm-like protein Hfq is required to promote pairing between both RNAs when their sequence complementarity is limited. To provide a first global view on the post-transcriptional landscape of the ?-proteobacterium Caulobacter crescentus, we have identified the Hfq-binding RNAs employing High-throughput sequencing of RNA isolated by cross-linking immunoprecipitation (HITS-CLIP). A total of 261 RNAs, including 3 unannotated RNAs, were successfully identified and classified according to putative function. Moreover, possible interactions between the identified sRNAs with mRNA targets were postulated through computational target predictions.

SUBMITTER: Assis NG

PROVIDER: S-EPMC6546397 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Identification of Hfq-binding RNAs in Caulobacter crescentus.

Assis Nadine G NG Ribeiro Rodolfo A RA da Silva Larissa G LG Vicente Alexandre M AM Hug Isabelle I Marques Marilis V MV

RNA biology 20190326 6

Small RNAs are important for post-transcriptional regulation of gene expression, affecting stability and activity of their target mRNAs. The bacterial Sm-like protein Hfq is required to promote pairing between both RNAs when their sequence complementarity is limited. To provide a first global view on the post-transcriptional landscape of the α-proteobacterium Caulobacter crescentus, we have identified the Hfq-binding RNAs employing High-throughput sequencing of RNA isolated by cross-linking immu ...[more]

PMID: 30870072

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