Identifying cis Elements for Spatiotemporal Control of Mammalian DNA Replication.
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ABSTRACT:
SUBMITTER: Sima J
PROVIDER: S-EPMC6546437 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Publications
Identifying cis Elements for Spatiotemporal Control of Mammalian DNA Replication.
Sima Jiao J Chakraborty Abhijit A Dileep Vishnu V Michalski Marco M Klein Kyle N KN Holcomb Nicolas P NP Turner Jesse L JL Paulsen Michelle T MT Rivera-Mulia Juan Carlos JC Trevilla-Garcia Claudia C Bartlett Daniel A DA Zhao Peiyao A PA Washburn Brian K BK Nora Elphège P EP Kraft Katerina K Mundlos Stefan S Bruneau Benoit G BG Ljungman Mats M Fraser Peter P Ay Ferhat F Gilbert David M DM
Cell 20181227 4
The temporal order of DNA replication (replication timing [RT]) is highly coupled with genome architecture, but cis-elements regulating either remain elusive. We created a series of CRISPR-mediated deletions and inversions of a pluripotency-associated topologically associating domain (TAD) in mouse ESCs. CTCF-associated domain boundaries were dispensable for RT. CTCF protein depletion weakened most TAD boundaries but had no effect on RT or A/B compartmentalization genome-wide. By contrast, delet ...[more]
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