Ontology highlight
ABSTRACT:
SUBMITTER: Wachutka L
PROVIDER: S-EPMC6548502 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
eLife 20190426
RNA splicing is an essential part of eukaryotic gene expression. Although the mechanism of splicing has been extensively studied in vitro, in vivo kinetics for the two-step splicing reaction remain poorly understood. Here, we combine transient transcriptome sequencing (TT-seq) and mathematical modeling to quantify RNA metabolic rates at donor and acceptor splice sites across the human genome. Splicing occurs in the range of minutes and is limited by the speed of RNA polymerase elongation. Splici ...[more]