Dataset Information

Large-scale plasma metabolome analysis reveals alterations in HDL metabolism in migraine.

ABSTRACT:

Objective

To identify a plasma metabolomic biomarker signature for migraine.

Methods

Plasma samples from 8 Dutch cohorts (n = 10,153: 2,800 migraine patients and 7,353 controls) were profiled on a ¹H-NMR-based metabolomics platform, to quantify 146 individual metabolites (e.g., lipids, fatty acids, and lipoproteins) and 79 metabolite ratios. Metabolite measures associated with migraine were obtained after single-metabolite logistic regression combined with a random-effects meta-analysis performed in a nonstratified and sex-stratified manner. Next, a global test analysis was performed to identify sets of related metabolites associated with migraine. The Holm procedure was applied to control the family-wise error rate at 5% in single-metabolite and global test analyses.

Results

Decreases in the level of apolipoprotein A1 (β -0.10; 95% confidence interval [CI] -0.16, -0.05; adjusted p = 0.029) and free cholesterol to total lipid ratio present in small high-density lipoprotein subspecies (HDL) (β -0.10; 95% CI -0.15, -0.05; adjusted p = 0.029) were associated with migraine status. In addition, only in male participants, a decreased level of omega-3 fatty acids (β -0.24; 95% CI -0.36, -0.12; adjusted p = 0.033) was associated with migraine. Global test analysis further supported that HDL traits (but not other lipoproteins) were associated with migraine status.

Conclusions

Metabolic profiling of plasma yielded alterations in HDL metabolism in migraine patients and decreased omega-3 fatty acids only in male migraineurs.

SUBMITTER: Onderwater GLJ

PROVIDER: S-EPMC6550500 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Large-scale plasma metabolome analysis reveals alterations in HDL metabolism in migraine.

Onderwater Gerrit L J GLJ Ligthart Lannie L Bot Mariska M Demirkan Ayse A Fu Jingyuan J van der Kallen Carla J H CJH Vijfhuizen Lisanne S LS Pool René R Liu Jun J Vanmolkot Floris H M FHM Beekman Marian M Wen Ke-Xin KX Amin Najaf N Thesing Carisha S CS Pijpers Judith A JA Kies Dennis A DA Zielman Ronald R de Boer Irene I van Greevenbroek Marleen M J MMJ Arts Ilja C W ICW Milaneschi Yuri Y Schram Miranda T MT Dagnelie Pieter C PC Franke Lude L Ikram M Arfan MA Ferrari Michel D MD Goeman Jelle J JJ Slagboom P Eline PE Wijmenga Cisca C Stehouwer Coen D A CDA Boomsma Dorret I DI van Duijn Cornelia M CM Penninx Brenda W BW 't Hoen Peter A C PAC Terwindt Gisela M GM van den Maagdenberg Arn M J M AMJM

Neurology 20190403 16

<h4>Objective</h4>To identify a plasma metabolomic biomarker signature for migraine.<h4>Methods</h4>Plasma samples from 8 Dutch cohorts (n = 10,153: 2,800 migraine patients and 7,353 controls) were profiled on a <sup>1</sup>H-NMR-based metabolomics platform, to quantify 146 individual metabolites (e.g., lipids, fatty acids, and lipoproteins) and 79 metabolite ratios. Metabolite measures associated with migraine were obtained after single-metabolite logistic regression combined with a random-effe ...[more]

PMID: 30944236

Similar Datasets

Project description:BackgroundMyopia is one of the most common eye diseases leading to blurred distance vision. Inflammatory diseases could trigger or exacerbate myopic changes. Although gut microbiota bacteria are associated with various inflammatory diseases, little is known about its role in myopia.Materials and methodsThe mice were randomly divided into control and model groups, with the model group being attached-30D lens onto the eyes for 3 weeks. Then, mouse cecal contents and plasma were collected to analyze their intestinal microbiota and plasma metabolome.ResultsWe identified that the microbial composition differed considerably between the myopic and non-myopic mice, with the relative abundance of Firmicutes phylum decreased obviously while that of Actinobacteria phylum was increased in myopia. Furthermore, Actinobacteria and Bifidobacterium were positively correlated with axial lengths (ALs) of eyeballs while negatively correlated with refractive diopters. Untargeted metabolomic analysis identified 141 differentially expressed metabolites, and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed considerable enrichment mainly in amino acid metabolism pathways. Notably, pathways involved glutamate metabolism including "Glutamine and D-glutamate metabolism" and "Alanine, aspartate and glutamate metabolism" was changed dramatically, which presented as the concentrations of L-Glutamate and L-Glutamine decreased obviously in myopia. Interestingly, microbiome dysbiosis and metabolites alternations in myopia have a disrupting gut barrier feature. We further demonstrated that the gut barrier function was impaired in myopic mice manifesting in decreased expression of Occludin, ZO-1 and increased permeation of FITC-dextran.DiscussionMyopic mice had obviously altered gut microbiome and metabolites profiles compared to non-myopic mice. The dysbiosis and plasma metabolomics shift in myopia had an interrupting gut barrier feature. Our study provides new insights into the possible role of the gut microbiota in myopia and reinforces the potential feasibility of microbiome-based therapies in myopia.

Dataset Information

Large-scale plasma metabolome analysis reveals alterations in HDL metabolism in migraine.

Objective

Methods

Results

Conclusions

Publications

Large-scale plasma metabolome analysis reveals alterations in HDL metabolism in migraine.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets