Dataset Information

Ornithodoros (Ornithodoros) huajianensis sp. nov. (Acari, argasidae), a new tick species from the Mongolian marmot (Marmota bobak sibirica), Gansu province in China.

ABSTRACT: A new argasid species, belonging to the subgenus Ornithodoros, namely, Ornithodoros (Ornithodoros) huajianensis was described for the first time based on the females, males and nymphs. The morphological features of each stage in the life cycle are unique, making identification easy, but are similar to other species of the subgenus Ornithodoros. The new species was diagnosed by the broad rectangular tongue and triangular tongue-shaped posterior lip in the male genital apron, a shallow camerostome with definite folds and smaller mammillae with single seta mixed with larger ones in nymph and adults. The new species had been collected from the Mongolian marmots Marmota bobak sibirica in Huajian village, Gulang county, Gansu province, China. Data on the phylogenic position, hosts and geographic distribution are also provided.

SUBMITTER: Sun Y

PROVIDER: S-EPMC6551375 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Ornithodoros (Ornithodoros) huajianensis sp. nov. (Acari, argasidae), a new tick species from the Mongolian marmot (Marmota bobak sibirica), Gansu province in China.

Sun Yi Y Xu Rongman R Liu Zengjia Z Wu Mingyu M Qin Tong T

International journal for parasitology. Parasites and wildlife 20190507

A new argasid species, belonging to the subgenus Ornithodoros, namely, Ornithodoros (Ornithodoros) huajianensis was described for the first time based on the females, males and nymphs. The morphological features of each stage in the life cycle are unique, making identification easy, but are similar to other species of the subgenus Ornithodoros. The new species was diagnosed by the broad rectangular tongue and triangular tongue-shaped posterior lip in the male g ...[more]

PMID: 31194100

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Project description:Two Borrelia isolates (CA434 and CA435) cultured from the soft tick Ornithodoros coriaceus were analyzed by contour-clamped homogeneous electric field gel electrophoresis of unrestricted and ApaI-restricted DNA, standard electrophoresis of BamHI- and HindIII-restricted DNA, Southern hybridization, restriction fragment length polymorphism and sequencing of the 16S rRNA gene, and amplification of the 5S-23S intergenic spacer region. These isolates were compared with Borrelia coriaceae type strain Co53, B. burgdorferi sensu stricto strain CA4, and the relapsing-fever spirochete B. parkeri (undesignated). The 16S rRNA region of CA434 and CA435 differed from that of B. coriaceae type strain Co53 by the presence of 1 base (C) at position 367 (GenBank accession no. U42286). The linear plasmid profile of CA434 was similar to that of Co53, and the ApaI, BamHI, and HindIII restriction fingerprints of the total cellular DNA of CA434 and Co53 were similar. In contrast, CA435 differed somewhat from CA434 and Co53, which demonstrates that B. coriaceae is genetically diverse. Southern hybridization showed that the DNAs of CA434 and CA435 hybridized strongly with the digoxigenin-labeled DNA of Co53. Low homology was found between the DNA of Co53 and that of B. parkeri. The 16S rRNA sequence of B. parkeri was identical to previously published results for B. parkeri strain M3001 (GenBank accession number U42296). CA434 and CA435 represent only the second and third isolates of B. coriaceae obtained from any source since its initial isolation from an O. coriaceus tick in 1985. All three B. coriaceae isolates were derived from adult ticks collected from the same locality in northwestern California. Difficulties encountered in detecting B. coriaceae in, and isolating this spirochete from, the tissues of O. coriaceus are discussed. The lack of concordance between different detection or isolation methods suggests that reliance upon a single technique may grossly underestimate the true prevalence of spirochetal infection in wild-caught O. coriaceus ticks.

Project description:Anaplasma marginale is a Gram-negative, obligate intraerythrocytic bacterium that causes bovine anaplasmosis. While hard ticks of the genera Dermacentor and Rhipicephalus can be biological vectors, transmitting this pathogen via saliva during blood meals, blood-sucking insects, and fomites play a role as mechanical vectors. Little is known about the interaction between Anaplasma marginale and Argasidae ticks. Among soft ticks, Ornithodoros fonsecai (Labruna and Venzal) and Ornithodoros brasiliensis Aragão inhabit environments surrounding localities where many cases of bovine anaplasmosis have been reported. Ticks of the species O. fonsecai parasitize bats, while O. brasiliensis can parasitize different vertebrate species. Therefore, the present study aimed to feed third-instar nymphs artificially (N3) of O. fonsecai and O. brasiliensis using blood samples obtained from a calf naturally infected with A. marginale and rabbit blood added to A. marginale-containing bovine erythrocytes, to investigate the ability of these nymphs to acquire, infect and transstadially perpetuate this agent. For the artificial feeding system, adapted chambers and parafilm membranes were used. Nymphs of both tick species were submitted to different replications weighed before and after each feeding. Blood samples and molted ticks were submitted to DNA extraction, quantitative real-time PCR for the msp1β gene to detect A. marginale DNA, while a semi-nested polymerase chain reaction for the msp1α gene was performed for genotyping. Using calf blood naturally infected with A. marginale, among the three artificial feeding replications performed with O. fonsecai and O. brasiliensis nymphs, the DNA of A. marginale was detected in both nymphs after 30-50 days of molting. For artificial feeding with rabbit blood added to bovine erythrocytes containing A. marginale, the DNA of this pathogen was also detected in both nymph species. As for the assay for the msp1α gene, strains were found Is9; 78 24-2; 25; 23; α; and β. It was concluded that nymphs (N3) of O. fonsecai and O. brasiliensis could feed artificially through a parafilm membrane using blood from calves and rabbits infected by A. marginale. The DNA of A. marginale was detected in nymphs fed artificially of both tick species studied after molt. However, further studies are needed to confirm transstadial perpetuation in other instars and their host transmission capacity.

Dataset Information

Ornithodoros (Ornithodoros) huajianensis sp. nov. (Acari, argasidae), a new tick species from the Mongolian marmot (Marmota bobak sibirica), Gansu province in China.

Publications

<i>Ornithodoros</i> (<i>Ornithodoros</i>) <i>huajianensis</i> sp. nov. (Acari, argasidae), a new tick species from the Mongolian marmot (<i>Marmota bobak sibirica</i>), Gansu province in China.

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