Fitness Effects of Single Amino Acid Insertions and Deletions in TEM-1 β-Lactamase.

ABSTRACT: Short insertions and deletions (InDels) are a common type of mutation found in nature and a useful source of variation in protein engineering. InDel events have important consequences in protein evolution, often opening new pathways for adaptation. However, much less is known about the effects of InDels compared to point mutations and amino acid substitutions. In particular, deep mutagenesis studies on the distribution of fitness effects of mutations have focused almost exclusively on amino acid substitutions. Here, we present a near-comprehensive analysis of the fitness effects of single amino acid InDels in TEM-1 β-lactamase. While we found InDels to be largely deleterious, partially overlapping deletion-tolerant and insertion-tolerant regions were observed throughout the protein, especi

SUBMITTER: Gonzalez CE 

PROVIDER: S-EPMC6554054 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature