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New UPLC-MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients.

ABSTRACT:

Aim

A rapid UPLC-MS/MS method for the determination of tamoxifen (TAM), N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation.

Materials and methods

The analysis was achieved on a C¹⁸ analytical column, using a gradient elution with a mobile phase of water and acetonitrile for 4.5 min.

Results

The validated method demonstrated good linearity between 1 and 500 ng/ml for TAM and N-desmethyltamoxifen; between 0.2 and 100 ng/ml for endoxifen and between 0.1 and 50 ng/ml for 4-hydroxytamoxifen. The method also provided satisfactory results in terms of within day and between day imprecisions and accuracy, and also in terms of time stability and specificity.

Conclusion

The method is applied routinely for TAM monitoring from patients undergoing therapy.

SUBMITTER: Bobin-Dubigeon C

PROVIDER: S-EPMC6554689 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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New UPLC-MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients.

Bobin-Dubigeon Christine C Campone Mario M Rossignol Elsa E Salaun Estelle E Amiand Marie-Bernadette MB Bard Jean-Marie JM

Future science OA 20190322 5

<h4>Aim</h4>A rapid UPLC-MS/MS method for the determination of tamoxifen (TAM), <i>N</i>-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation.<h4>Materials and methods</h4>The analysis was achieved on a C<sup>18</sup> analytical column, using a gradient elution with a mobile phase of water and acetonitrile for 4.5 min.<h4>Results</h4>The validated method demonstrated good linearity between 1 and 500 ng/ml for TAM and <i>N</i>-de ...[more]

PMID: 31245038

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