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New UPLC-MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients.

ABSTRACT: Aim:A rapid UPLC-MS/MS method for the determination of tamoxifen (TAM), N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation. Materials and methods:The analysis was achieved on a C18 analytical column, using a gradient elution with a mobile phase of water and acetonitrile for 4.5 min. Results:The validated method demonstrated good linearity between 1 and 500 ng/ml for TAM and N-desmethyltamoxifen; between 0.2 and 100 ng/ml for endoxifen and between 0.1 and 50 ng/ml for 4-hydroxytamoxifen. The method also provided satisfactory results in terms of within day and between day imprecisions and accuracy, and also in terms of time stability and specificity. Conclusion:The method is applied routinely for TAM monitoring from patients undergoing therapy.

SUBMITTER: Bobin-Dubigeon C

PROVIDER: S-EPMC6554689 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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New UPLC-MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients.

Bobin-Dubigeon Christine C Campone Mario M Rossignol Elsa E Salaun Estelle E Amiand Marie-Bernadette MB Bard Jean-Marie JM

Future science OA 20190322 5

<h4>Aim</h4>A rapid UPLC-MS/MS method for the determination of tamoxifen (TAM), <i>N</i>-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation.<h4>Materials and methods</h4>The analysis was achieved on a C<sup>18</sup> analytical column, using a gradient elution with a mobile phase of water and acetonitrile for 4.5 min.<h4>Results</h4>The validated method demonstrated good linearity between 1 and 500 ng/ml for TAM and <i>N</i>-de ...[more]

PMID: 31245038

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