High-throughput identification of dominant negative polypeptides in yeast.
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ABSTRACT:
SUBMITTER: Dorrity MW
PROVIDER: S-EPMC6555411 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Publications
High-throughput identification of dominant negative polypeptides in yeast.
Dorrity Michael W MW Queitsch Christine C Fields Stanley S
Nature methods 20190408 5
Dominant negative polypeptides can inhibit protein function by binding to a wild-type subunit or by titrating a ligand. Here we use high-throughput sequencing of libraries composed of fragments of yeast genes to identify polypeptides that act in a dominant negative manner, in that they are depleted during cell growth. The method can uncover numerous inhibitory polypeptides for a protein and thereby define small inhibitory regions, even pinpointing individual residues with critical functional rol ...[more]
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