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Full-field structured-illumination super-resolution X-ray transmission microscopy.

ABSTRACT: Modern transmission X-ray microscopy techniques provide very high resolution at low and medium X-ray energies, but suffer from a limited field-of-view. If sub-micrometre resolution is desired, their field-of-view is typically limited to less than one millimetre. Although the field-of-view increases through combining multiple images from adjacent regions of the specimen, so does the required data acquisition time. Here, we present a method for fast full-field super-resolution transmission microscopy by structured illumination of the specimen. This technique is well-suited even for hard X-ray energies above 30?keV, where efficient optics are hard to obtain. Accordingly, investigation of optically thick specimen becomes possible with our method combining a wide field-of-view spanning multiple millimetres, or even centimetres, with sub-micron resolution and hard X-ray energies.

SUBMITTER: Gunther B

PROVIDER: S-EPMC6555788 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Full-field structured-illumination super-resolution X-ray transmission microscopy.

Günther Benedikt B Hehn Lorenz L Jud Christoph C Hipp Alexander A Dierolf Martin M Pfeiffer Franz F

Nature communications 20190607 1

Modern transmission X-ray microscopy techniques provide very high resolution at low and medium X-ray energies, but suffer from a limited field-of-view. If sub-micrometre resolution is desired, their field-of-view is typically limited to less than one millimetre. Although the field-of-view increases through combining multiple images from adjacent regions of the specimen, so does the required data acquisition time. Here, we present a method for fast full-field super-resolution transmission microsc ...[more]

PMID: 31175291

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