M⁶A methyltransferase METTL3 suppresses colorectal cancer proliferation and migration through p38/ERK pathways.

ABSTRACT: Purpose: Although many biological processes are involved in the modification of N⁶-methyladenosine (m⁶A), the exact role of m⁶A in the development of malignant tumors remains unclear. Methyltransferase 3 (METTL3) is a major RNA N⁶-methyladenosine methyltransferase. We aimed to explore the role of METTL3 in colorectal cancer (CRC) carcinogenesis and disease progression. Methods: In this study, immunohistochemistry was performed with a tissue microarray. qRT-PCR and Western blots were used to evaluate the expression of METTL3 in CRC cells. The effect of METTL3 on cell proliferation, migration and invasion of CRC cells was examined by IncuCyte Live Cell Analysis System and transwell assay, respectively. Results: The results sugg

SUBMITTER: Deng R 

PROVIDER: S-EPMC6556107 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature