Dataset Information

Characterization of internalin genes in Listeria monocytogenes from food and humans, and their association with the invasion of Caco-2 cells.

ABSTRACT: Background:Internalins are surface proteins that are utilized by Listeria monocytogenes to facilitate its invasion into human intestinal epithelial cells. The expression of a full-length InlA is one of essential virulence factors for L. monocytogenes to cross the intestinal barrier in order to invade epithelial cells. Results:In this study, the gene sequences of inlA in 120 L. monocytogenes isolates from food (n?=?107) and humans (n?=?13) were analyzed. Premature stop codon (PMSC) mutations in inlA were identified in 51 isolates (50 from food and 1 from human). Six mutation types of PMSCs were identified. Among the 51 isolates with PMSCs in inlA, there were 44 serogroup 1/2c, 3c isolates from food, of which seven belonged to serogroups 1/2a, 3a. A total of 153,382 SNPs in 2247 core genes from 42 genomes were identified and used to construct a phylogenetic tree. Serotype 1/2c isolates with inlA PMSC mutations were grouped together. Cell culture studies on 21 isolates showed that the invasion to Caco-2 cells was significantly reduced among isolates with inlA PMSC mutations compared to those without PMSC mutations (P?

SUBMITTER: Su X

PROVIDER: S-EPMC6558679 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Characterization of internalin genes in <i>Listeria monocytogenes</i> from food and humans, and their association with the invasion of Caco-2 cells.

Su Xudong X Cao Guojie G Zhang Jianmin J Pan Haijian H Zhang Daofeng D Kuang Dai D Yang Xiaowei X Xu Xuebin X Shi Xianming X Meng Jianghong J

Gut pathogens 20190610

<h4>Background</h4>Internalins are surface proteins that are utilized by <i>Listeria monocytogenes</i> to facilitate its invasion into human intestinal epithelial cells. The expression of a full-length InlA is one of essential virulence factors for <i>L. monocytogenes</i> to cross the intestinal barrier in order to invade epithelial cells.<h4>Results</h4>In this study, the gene sequences of inlA in 120 <i>L. monocytogenes</i> isolates from food (n = 107) and humans (n = 13) were analyzed. Premat ...[more]

PMID: 31198443

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Project description:BACKGROUND:Listeria monocytogenes is a widespread foodborne pathogen that can cause listeriosis, a potentially fatal infection. L. monocytogenes is subdivided into four phylogenetic lineages, with the highest incidence of listeriosis occurring within lineage I followed by lineage II. Strains of L. monocytogenes differ in their phenotypic characteristics, including virulence. However, the genetic bases for these observed differences are not well understood, and current efforts to monitor L. monocytogenes in food consider all strains to be equally virulent. We use a comparative genomics approach to identify genes and single nucleotide polymorphisms (SNPs) in 174 clinical and food isolates of L. monocytogenes that potentially contribute to virulence or the capacity to adapt to food environments. RESULTS:No SNPs are significantly associated with food or clinical isolates. No genes are significantly associated with food or clinical isolates from lineage I, but eight genes consisting of multiple homologues are associated with lineage II food isolates. These include three genes which encode hypothetical proteins, the cadmium resistance genes cadA and cadC, the multi-drug resistance gene ebrB, a quaternary ammonium compound resistance gene qac, and a regulatory gene. All eight genes are plasmid-borne, and most closed L. monocytogenes plasmids carry at least five of the genes (24/27). In addition, plasmids are more frequently associated with lineage II food isolates than with lineage II clinical isolates. CONCLUSIONS:We identify eight genes that are significantly associated with food isolates in lineage II. Interestingly, the eight genes are virtually absent in lineage II outbreak isolates, are composed of homologues which show a nonrandom distribution among lineage I serotypes, and the sequences are highly conserved across 27 closed Listeria plasmids. The functions of these genes should be explored further and will contribute to our understanding of how L. monocytogenes adapts to the host and food environments. Moreover, these genes may also be useful as markers for risk assessment models of either pathogenicity or the ability to proliferate in food and the food processing environment.

Dataset Information

Characterization of internalin genes in Listeria monocytogenes from food and humans, and their association with the invasion of Caco-2 cells.

Publications

Characterization of internalin genes in <i>Listeria monocytogenes</i> from food and humans, and their association with the invasion of Caco-2 cells.

Similar Datasets

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