Ontology highlight
ABSTRACT:
SUBMITTER: Puchalska P
PROVIDER: S-EPMC6559243 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Puchalska Patrycja P Martin Shannon E SE Huang Xiaojing X Lengfeld Justin E JE Daniel Bence B Graham Mark J MJ Han Xianlin X Nagy Laszlo L Patti Gary J GJ Crawford Peter A PA
Cell metabolism 20181115 2
Metabolic plasticity has been linked to polarized macrophage function, but mechanisms connecting specific fuels to tissue macrophage function remain unresolved. Here we apply a stable isotope tracing, mass spectrometry-based untargeted metabolomics approach to reveal the metabolome penetrated by hepatocyte-derived glucose and ketone bodies. In both classically and alternatively polarized macrophages, [<sup>13</sup>C]acetoacetate (AcAc) labeled ∼200 chemical features, but its reduced form D-[<sup ...[more]