Vascular endothelial PDPK1 plays a pivotal role in the maintenance of pancreatic beta cell mass and function in adult male mice.

ABSTRACT:

Aims/hypothesis

The aim of this study was to elucidate the impact of 3'-phosphoinositide-dependent protein kinase-1 (PDPK1) in vascular endothelial cells on the maintenance of pancreatic beta cell mass and function.

Methods

Male vascular endothelial cell-specific Pdpk1-knockout mice (Tie2^+/-/Pdpk1^flox/flox mice) and their wild-type littermates (Tie2^-/-/Pdpk1^flox/flox mice; control) were used for this study. At 12 weeks of age, an IPGTT and OGTT were conducted. Pancreatic blood flow was measured under anaesthesia. Thereafter, islet blood flow was measured by the microsphere method. Mice were killed for islet isolation and further functional study and mRNA was extracted from islets. Pancreases were sampled for immunohistochemical ana

SUBMITTER: Obata A 

PROVIDER: S-EPMC6560212 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature