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Universal light-sheet generation with field synthesis.

ABSTRACT: We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.

SUBMITTER: Chang BJ

PROVIDER: S-EPMC6561754 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Universal light-sheet generation with field synthesis.

Chang Bo-Jui BJ Kittisopikul Mark M Dean Kevin M KM Roudot Philippe P Welf Erik S ES Fiolka Reto R

Nature methods 20190225 3

We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets ...[more]

PMID: 30804550

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